Transcription profiling of B7.5 lineage cells in the tunicate Ciona intestinalis to study cardiac cell migration

ABSTRACT: Eight different types of sample (three samples each) were used in this study of cardiac cell migration in the tunicate Ciona intestinalis. The B7.5 lineage cells were isolated by Fluorescent Activated Cell Sorting (FACS) at the tailbud stage, when the cardiac precursors (anterior B7.5 lineage cells) are migrating into the trunk, while their sister cells (posterior 7.5 lineage cells) remain in the tail, where they form muscle cells. Cells were sorted at the late gastrula stage, prior to migration induction and after manipulation of the FGF-MAPK-Ets signaling pathway (targeted expression of dominant negative FGF receptor and Ets:VP16 fusion protein, targeted expression was achieved using the Mesp cis-regulatory DNA), FoxF function (a direct target of FGF-MAPK-Ets signaling, required primaril

ORGANISM(S): Ciona intestinalis

SUBMITTER: Lionel Christiaen 

PROVIDER: E-MEXP-1478 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress