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Chromatin immunoprecipitation to determine MSL1, MSL3, MOF and H4K16Ac binding in Drosophila SL2 and Kc cells reveals MOF as a key regulator of dosage compensation and gene expression in Drosophila

ABSTRACT: Genome-wide binding profiles of MSL1, MSL3, MOF and H4K16Ac in Drosophila SL2 and Kc cells.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Asifa Akhtar

PROVIDER: E-MEXP-1508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis reveals MOF as a key regulator of dosage compensation and gene expression in Drosophila.

Kind Jop J Vaquerizas Juan M JM Gebhardt Philipp P Gentzel Marc M Luscombe Nicholas M NM Bertone Paul P Akhtar Asifa A

Cell 20080501 5

Dosage compensation, mediated by the MSL complex, regulates X-chromosomal gene expression in Drosophila. Here we report that the histone H4 lysine 16 (H4K16) specific histone acetyltransferase MOF displays differential binding behavior depending on whether the target gene is located on the X chromosome versus the autosomes. More specifically, on the male X chromosome, where MSL1 and MSL3 are preferentially associated with the 3' end of dosage compensated genes, MOF displays a bimodal distributio ...[more]

PMID: 18510926

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