Genotyping of S. aureus SH1000 transposon mutants to identify mutant positions
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ABSTRACT: A library of transposon mutants were generated in S.aureus strain SH1000 using a custom Mariner transposon with an outward-facing T7 promoter. Genomic DNA was extracted from the library and digested with an appropriate restriction enzyme (AluI or RsaI). Labelled RNA run-offs were produced from the T7 promoter and hybridised to a tiling microarray to determine the position of the mutant. This was done using a library of a million S. aureus mutants, and genes not disrupted by a transposon were inferred to be essential for replication and growth in vitro.
ORGANISM(S): Staphylococcus aureus
SUBMITTER: Roy Chaudhuri
PROVIDER: E-MEXP-1588 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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