Transcription profiling of Paxgene collected human whole blood and hematopoietic lineages
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ABSTRACT: Assessment of transcriptome stability in Paxgene collected whole blood and representation of hematopoietic lineages of whole blood transcriptome using single primer isothermal amplification probes without globin reduction protocols. As well as the stability assessment of Paxgene collected samples we investigated whether expression profiling of whole blood RNA (without globin reduction procedures) using the NuGEN single-primer isothermal amplification methods (which generate single stranded cDNA) allows faithful representation of individual lineages. ie. are we losing a lot of information by looking at whole blood rather than PBMCs or individual lineages. These 7 cell lineages were not stored in Paxgene tubes and are only for the purpose of querying the data generated from the Paxgene/sscDNA dataset (12 samples) in order to determine the representation of individual lineage transcriptomes in that dataset.
ORGANISM(S): Homo sapiens
SUBMITTER: Gino Miele
PROVIDER: E-MEXP-1600 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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