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Transcription profiling of zebrafish embryos injected with Ofd1 morphlino compared to wild type

ABSTRACT: Comparison of Ofd1 morphlino injected zebrafish embryos with wild type

ORGANISM(S): Danio rerio

SUBMITTER: John Collins

PROVIDER: E-MEXP-1879 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Convergent extension movements and ciliary function are mediated by ofd1, a zebrafish orthologue of the human oral-facial-digital type 1 syndrome gene.

Ferrante Maria I MI Romio Leila L Castro Silvia S Collins John E JE Goulding David A DA Stemple Derek L DL Woolf Adrian S AS Wilson Stephen W SW

Human molecular genetics 20081029 2

In humans, OFD1 is mutated in oral-facial-digital type I syndrome leading to prenatal death in hemizygous males and dysmorphic faces and brain malformations, with polycystic kidneys presenting later in life in heterozygous females. To elucidate the function of Ofd1, we have studied its function during zebrafish embryonic development. In wild-type embryos, ofd1 mRNA is widely expressed and Ofd1-green fluorescent protein (GFP) fusion localizes to the centrosome/basal body. Disrupting Ofd1 using an ...[more]

PMID: 18971206

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