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Transcription profiling of human 293T cells transfected with HIPK2 or an empty vector and exposed to either hypoxia or normoxia conditions

ABSTRACT: To determine the consequences of hypoxia-mediated HIPK2 clearance on gene expression, cells 293T were transfected with either the HIPK2 expression vector or the corresponding empty vector and then subjected to either hypoxia conditions (2% O2 for 6 h) or normoxia conditions. RNA total was extracted and later a study of the different gene expression under the four conditions detailed above was performed. The correct expression of HIPK2 was assessed by western blot in a small cell pool. The quality of RNA was quantified before the array study.

ORGANISM(S): Homo sapiens

SUBMITTER: Elena Puccetti

PROVIDER: E-MEXP-1896 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An inducible autoregulatory loop between HIPK2 and Siah2 at the apex of the hypoxic response.

Calzado Marco A MA de la Vega Laureano L Möller Andreas A Bowtell David D L DD Schmitz M Lienhard ML

Nature cell biology 20081130 1

Oxygen deprivation (hypoxia) results in reprogrammed gene expression patterns that induce multifaceted cellular responses. Here we identify a regulated interaction between the serine/threonine kinase HIPK2 and the ubiquitin E3 ligase Siah2 as a mechanism controlling the hypoxic response. Under normoxic conditions, several mechanisms ensure HIPK2 stability: only a fraction of HIPK2 is found in association with Siah2, whereas HIPK2-mediated phosphorylation of this E3 ligase at positions 26, 28 and ...[more]

PMID: 19043406

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