Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of replication checkpoint response in fission yeast

ABSTRACT: Transcription profiling of replication checkpoint response in fission yeast.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Adam Rosebrock

PROVIDER: E-MEXP-1902 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The DNA replication checkpoint directly regulates MBF-dependent G1/S transcription.

Dutta Chaitali C Patel Prasanta K PK Rosebrock Adam A Oliva Anna A Leatherwood Janet J Rhind Nicholas N

Molecular and cellular biology 20080728 19

The DNA replication checkpoint transcriptionally upregulates genes that allow cells to adapt to and survive replication stress. Our results show that, in the fission yeast Schizosaccharomyces pombe, the replication checkpoint regulates the entire G(1)/S transcriptional program by directly regulating MBF, the G(1)/S transcription factor. Instead of initiating a checkpoint-specific transcriptional program, the replication checkpoint targets MBF to maintain the normal G(1)/S transcriptional program ...[more]

PMID: 18662996

Similar Datasets

Transcription profiling of yeast strains in which Hsk1-Dfp1 has been tethered near a replication origin

Project description:Transcription profiling of strains in which Hsk1-Dfp1 has been tethered near a replication origin.

2008-09-25 | E-MEXP-1811 | biostudies-arrayexpress

Transcription profiling time series of fission yeast after selective inhibition of Mcs6 and Cdk9

Project description:Selective inhibition of S. pombe Mcs6, Cdk9, or both via bulky nucleotide analogue.

2009-03-30 | E-MEXP-1746 | biostudies-arrayexpress

S. pombe oxidative stress response time course

Project description:S. pombe cells were challenged with 0.5 mM hydrogen peroxide for various periods of time (0, 10, 30, 60, 120, 180 minutes). Total RNA was isolated at each time point, labeled with Cy3 and hybridized on cDNA microarrays competitively with Cy5 labeled RNA pooled from all 6 samples. A biological replicate was performed in analyzed as a dyw swap (pool = Cy3, time series = Cy5).

2012-02-22 | E-MTAB-891 | biostudies-arrayexpress

Transcription profiling of budding yeast during meiosis to examine the effects of Cdc7p inactivation

Project description:Global effects of Cdc7p inactivation during early budding yeast meiosis

2008-10-02 | E-MEXP-1765 | biostudies-arrayexpress

The Fission Yeast RNA Binding Protein Mmi1 Regulates Meiotic Genes by Controlling Intron-Specific Splicing and Polyadenylation-Coupled RNA Turnover

Project description:Analysis of the transcription profiles of mutant strains that regulate meiotic genes in S. pombe.

2011-04-01 | E-MEXP-3039 | biostudies-arrayexpress

Pigeon lactation

Project description:comparison of lactating pigeon crop tissue with non lactating pigeon crop tissue

2011-08-31 | E-MEXP-3314 | biostudies-arrayexpress

Repression of meiotic genes by antisense transcription and by Fkh2 transcription factor in Schizosaccharomyces pombe

Project description:In S. pombe, about 5% of genes are meiosis-specific and accumulate little or no mRNA during vegetative growth. Here we use Affymetrix tiling arrays to characterize transcripts in vegetative and meiotic cells. In vegetative cells, many meiotic genes, especially those induced in mid-meiosis, have abundant antisense transcripts. These results suggest that antisense transcription represses sense transcription of meiotic genes in vegetative cells. Although the mechanism(s) of antisense mediated transcription repression need to be further explored, our data indicates that RNAi machinery, such as Rdp1, is not required for repression. Previously, we and others used non-strand specific methods to study splicing regulation of meiotic genes and concluded that 28 mid-meiotic genes are spliced only in meiosis. We now demonstrate that the “unspliced” signal in vegetative cells comes from the antisense RNA, not from unspliced sense RNA, andwe argue against the idea that splicing regulates these mid-meiotic genes. Most of these mid-meiotic genes are induced in mid-meiosis by the forkhead transcription factor Mei4. Interestingly, deletion of a different forkhead transcription factor, Fkh2, allows low levels of sense expression of some mid-meiotic genes in vegetative cells. We propose that expression of mid-meiotic genes is kept tightly off in vegetative cells by two independent ways: antisense transcription and Fkh2 repression.

2011-11-01 | E-MEXP-3414 | biostudies-arrayexpress

Transcription profiling of mouse midbrain biopsies of Nurr1 knockouts (3 pools) against 1 pool of controls

Project description:mouse midbrain biopsies of Nurr1 knockouts (3 pools) against 1 pool of controls

2009-06-10 | E-TABM-711 | biostudies-arrayexpress

Transcription profiling of procyclic Trypanosoma brucei cultured at 27oC or under heat shock stress conditions at 41oC

Project description:Procyclic trypanosomes (strain 427 lister) were grown in culture under standard conditions at 27ºC in SDM79 medium with 10% foetal bovine serume (Brun and Schnenberger, 1979), in a gazed incubator (5% CO2). Logarithmically growing procyclic cells (at about 5*10^6 cells/ml, at 27°C) were added to one volume medium that had been heated to 53°C and incubated at 41ºC for 60 minutes in a waterbath in a closed tube (41ºC sample). The control cells were added to one volume medium at 27ºC and also incubated for 60 minutes in a closed tube at 27°C. Cells were harvested and washed once in PBS. The harvesting was done within 8 minutes.

2008-09-10 | E-MEXP-1476 | biostudies-arrayexpress

Transcription profiling of human pancreas NS-928 cell lines modulated by COX-2

Project description:BxPc-3, Miapaca-2 and ASPC-1 cell lines were treated with 100 µM NS-398 in DMSO or with DMSO alone (control) for 48 h. Total-RNA from each sample was isolated with the RNeasy kit of Qiagen (Hilden, Germany) according to the manufacturer's protocol. The integrity of the isolated RNA was checked on an Agilent Bioanalyser 2100 using the RNA 6000 Nano Kit (Agilent Technologies, Palo Alto, USA) according to the manufacturer's protocol as recommended by the manufacturer. Microarrays were produced and processed as described before. Human cDNAs representing some 7,000 genes that are highly associated with the occurrence of pancreatic cancer including apoptotic and oncogenic genes, growth factors, angiogenic, cell cycle, metastasis-associated, and housekeeping genes were PCR-amplified using amino-modified M13 universal primers. PCR-products were purified with Multiscreen PCR (Millipore, Schwalbach, Germany), resuspended in spotting solution (TeleChem International, Sunnyvale, USA) and arrayed onto slides with epoxysilane surface (Quantifoil Micro Tools, Jena, Germany). DNA spotting was done with a Micro-Arrayer of Engineering Services Inc. (Virtek's arrayer system, BioRad, Munich, Germany) using SMP3 pins (TeleChem). Transcript profiling Fluorescently labelled cDNA samples were prepared from 15 µg total-RNA and Cy3- or Cy5-labelled dCTP was directly incorporated during first-strand synthesis . Hybridisation to the microarrays was done in hybridisation chambers (TeleChem) at 62°C overnight. After washing in 0.1xSSC, fluorescence signals were detected with a confocal ScanArray 5000 scanner (Packard Bioscience, USA) and analysed with GenePix Pro 6 (Axon Instruments, Union City, USA).

2010-07-06 | E-MEXP-1870 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data