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Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 inoculated onto pea seeds compared to cells grown in free-living culture on succinate ammonia AMS


ABSTRACT: Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.

ORGANISM(S): Rhizobium leguminosarum

SUBMITTER: philip poole 

PROVIDER: E-MEXP-1925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptomic analysis of Rhizobium leguminosarum biovar viciae in symbiosis with host plants Pisum sativum and Vicia cracca.

Karunakaran R R   Ramachandran V K VK   Seaman J C JC   East A K AK   Mouhsine B B   Mauchline T H TH   Prell J J   Skeffington A A   Poole P S PS  

Journal of bacteriology 20090417 12


Rhizobium leguminosarum bv. viciae forms nitrogen-fixing nodules on several legumes, including pea (Pisum sativum) and vetch (Vicia cracca), and has been widely used as a model to study nodule biochemistry. To understand the complex biochemical and developmental changes undergone by R. leguminosarum bv. viciae during bacteroid development, microarray experiments were first performed with cultured bacteria grown on a variety of carbon substrates (glucose, pyruvate, succinate, inositol, acetate, a  ...[more]

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