Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

52 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Arabidopsis mgo3 mutant leaves

ABSTRACT: Identification of molecular target of MGO3 genes. 2 first leaves of mgo3-2 mutant or wild-type plants (WS ecotype) were harvested from plants grown during 2 weeks in vitro on MS medium in long days conditions. 3 repeats have been done for each samples

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Lemonnier Gaëtan

PROVIDER: E-MEXP-196 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Arabidopsis Cul3a-1 mutant and control plants during germination in far red light

Project description:Parallel germination of Cul3a-1 mutant and control plants in far red light.

2005-01-01 | E-MEXP-175 | biostudies-arrayexpress

Transcription profiling of Arabidopsis strain with T-DNA insertions in the HAF2 gene

Project description:T-DNA insertions in the HAF2 gene are characterised. The mutation of the gene induced production of pale young leaves. Genetic analysis showed that HAF2 is required for both phytochrome and cryptochrome signalling. Accordingly, light-inducible gene expression and CAB2 promoter activity were affected by the mutation. The present transcriptome analysis showed that the mutation affected about 9% of Arabidopsis genes in 14 day-old leaves.

2004-10-05 | E-MEXP-178 | biostudies-arrayexpress

Transcription profiling of Arabidopsis Col-0 hypocotyls time series treated with isoxaben vs untreated samples

Project description:1. Study the molecular basis of the growth acceleration observed in hypocotyl cells. We previously have observed that cell elongation takes place in two distinct phases (Refregier et al., 2004). A slow growth phase during which a thick polylamellated wall is deposited and a rapid growth phase during which cell wall polymers are extensively remodelled. In dark-grown hypocotyls the slow growth phase takes place during the first 48h after seed-imbibition synchronously in all cells. At 48h after imbibition, cells at the basis of the hypocotyl undergo a growth acceleration, this acceleration follows an acropetal gradient along the hypocotyl. In this experiment, we investigated the changes in transcript abundance that accompany this sudden increase in growth rate. 2. Study the feed-back mechanisms involved in the coordination between cellulose synthesis and the cell elongation. The inhibition of cellulose using chemical inhibitors also inhibits cell elongation. In the same study (Refregier et al., 2004), we have observed that the effect of the cellulose synthesis inhibitor isoxaben on cell elongation is different dependent on the growth stage. When applied during the slow growth phase, cells continue to elongate slowly and do not show the growth acceleration at 48h after imbibition. Surprisingly, when applied after the growth acceleration, isoxaben does not inhibit subsequent growth. In this study we compared the effects of isoxaben on the transcript profiles before and after the growth acceleration. This should inform us about the response of the hypocotyl cells to the inhibition of cellulose and should provide insights into the molecular events that underly the observed coupling between cellulose synthesis and cell elongation.

2005-01-01 | E-MEXP-195 | biostudies-arrayexpress

Transcription profiling of Arabidopsis floral stem development

Project description:The aim of the experiment was to analyse the modification of the change in expression of genes encoding enzymes involved in the modification of cell wall polysaccharide structure during development.

2005-03-01 | E-MEXP-187 | biostudies-arrayexpress

Transcription profiling of wild type and atNRT2.7 genotype Arabidopsis leaves and roots grown in high or low nitrate conditions

Project description:Does the NRT2.7 participate to the HATS? We would like to understand the function of the AtNRT2.7 in nitrate transport under limiting and non limiting nitrate conditions. Plants were harvested after 32 days for plants under high nitrogen nutrition and 42 days for low nitrogen nutrition. Plants were grown hydroponic condition (short day, 170 uE).

2007-07-01 | E-MEXP-801 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type, AtVdac1 knock-outs, p26 knock-outs and AtVdac1-p26 double kock-outs in response to growth in medium with salicylic acid

Project description:Functions of AtVDAC1 and its partner p26 in Arabidopsis thaliana. We previously showed that knock-out mutants impaired in AtVdac1 and p26 (AtVdac1 protein partner) are hypersensitive to salicylic acid: the root growth is more inhibited in response to 30 uM salicylic acid (SA) compared to the wild type. We want to know now which genes are up- or down-regulated in the mutants in control and SA conditions. We want to compare the RNAm contents in the wild type, vdac1 and p26 mutants and, vdac1-p26 double mutant. One-week old plantlets were transferred for 2 hours on a fresh medium containing either 0 or 30uM salicylic acid. Arabidopsis lines (WT, vdac1.1, p26.1, vdac1.1-p26.1) were grown in vertical position on ABIS medium (Lanquar et al., 2006). One week-old seedlings were transferred on a fresh ABIS medium containing either 0 or 30 uM salicylic acid. After 2 hours, the all seedlings were harvested. Three replicates were done; total RNAs from these 3 experiments were mixed together for the transcriptomic analysis.

2007-06-19 | E-MEXP-795 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thioredoxin reductase knock-out mutant

Project description:Transcriptomic study of a thioredoxin reductase knock-out mutant. Biological question : Thioredoxins are small redox proteins implicated in crucial pathways of cell life. They are reduced by a conserved flavoprotein named NADPH-dependent thioredoxin reductase (NTR). In order to give an insight into the functions of the NTR/thioredoxin pathway in Arabidopsis, we have used a reverse genetic approch. Both NTR genes (NTRA and NTRB) have been inactivated in the double ntra ntrb mutant. T-DNA lines have been provided by the SALK library. Surprisingly, this mutant shows a limited phenotype suggested that some additional redox pathways are involved in compensating the inactivation of the NTR/thioredoxin pathway. In order to isolate compensatory genes that would be transcriptionally induced in the ntra ntrb mutant, we have performed this CATMA project. Seeds of the wild-type Col-0 ecotype and of the ntra ntrb homozygote double mutant were sowed in soil. Three replicates of lines were made (Col-0 1, 2 and 3 ntra ntrb 1, 2 and 3) : plants were put at different places of the greenhouse and grown at different times. Germination was synchronized by 4 days at 4C and plants were grown during 17 days, until they reached a 6 rosette leaves stage. Entire plants (30 plants per sample) were harvested, frozen in liquid nitrogen and stored at -80C until extraction.

2005-09-15 | E-MEXP-365 | biostudies-arrayexpress

Transcription profiling of Arabidopsis nin7-1 and nin7-2 in a low nitrogen regime

Project description:Does the ko of NIN7 leads to a differential expression in comparison to the wildtype. Can we repeat the results from the first experiment; and is there a difference between the two alleles? Plants were harvested during the first 3 hours of light after 42 days growth on 0.2mM or 6mM nitrate in hydroponic condition (short day, 170 uE).

2006-12-19 | E-MEXP-516 | biostudies-arrayexpress

Transcription profiling of Arabidopsis treated with seaweed extract vs those grown hydroponically

Project description:Columbia Arabidopsis ecotype were grown hydroponically on 1 mM NH4NO3 as sole nitrogen source during 35 days under short days. Plants leaves were then sprayed with a seaweed extract or with water (control). Root and shoot samples were harvested separately 1 hour and 7 days after this spraying.

2007-01-26 | E-MEXP-573 | biostudies-arrayexpress

Transcription profiling of Arabidopsis in standards condition and in response to nickel treatment to assess the effect nicotianamine over-accumulation on the transcriptome of A. thaliana

Project description:Effect of nicotianamine over-accumulation on the transcriptome of A. thaliana in standard condition and in response to nickel treatment After germination, plants were grown hydroponically for 6 weeks in classical nutrient solution (Marques et al, New Phytol, 2004, vol 164, pp 289-95) and then treated for 48 hours with 50 uM NiSO4 or with no nickel added for control. Biological question : Nicotianamine is a known metal chelator (of Mn, Fe, Zn, Ni, Cu) suspected to be involved in metal delivery and/or circulation in planta. This project aim at understanding the effect of the modulation of the nicotianamine content on the transcriptome of Arabidopsis thaliana in control hydroponic condition and in response to nickel treatment both at the root and at the leaf level.

2006-06-01 | E-MEXP-373 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data