Transcription profiling of mouse embyronic stem cells expressing the doxycycline-inducible Xist cDNA transgene inserted into chromosome 12 to study of Xist RNA mediated silencing

ABSTRACT: A doxycycline-inducible Xist cDNA transgene was introduced into mouse chromosome 12 at approximately 110Mb (NCBI build m36). Insertion of the transgene was heterozygous, so only one copy of chromosome 12 carried the transgene, and the other chromosome 12 was wild-type. Upon doxycycline induction, the transgene is expressed, producing Xist transgene RNA which localises to autosomal material in cis, leading to silencing of genes adjacent to the transgene. Due to the heterozygous nature of transgene insertion and the cis nature of silencing, for any silenced gene, the maximum possible downregulation in expression levels was 50%. To quantify the changes in the levels of gene expression in response to Xist transgene expression, total RNA was extracted from differentiated ES cells treated

ORGANISM(S): Mus musculus

SUBMITTER: Amy Tang 

PROVIDER: E-MEXP-2088 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress