Transcription profiling of the Arctic springtail Megaphorura arctica after cold or salt dehydration to study insect cyroprotective dehydration

ABSTRACT: The 13,824 cDNA microarray was constructed by printing 6912 PCR-amplified cDNA clones in duplicate. These were derived from a previous EST study (Clark et al, 2007) and comprised 3840 clones from the Library D2 (fully dehydrated animals, cooled to -14°C) and 3072 clones from the Library D1 (dehydrated animals cooled to -2°C). The Stratagene SpotReport Alien Array Validation System (Stratagene, La Jolla, CA, USA) was included on the microarray. Construction and hybridization of the arrays were performed as described in Purac et al (2008) with the exception of the amino-modified primers used for the initial cDNA amplification for array printing, which in this study were: (pAL32FOR: TTCTCGGGAAGCGCG and M13 forward: GTAAAACGACGGCCAG). Hybridizations were performed using control animals in combination with the groups listed below.



Six groups of animals were used for the hybridizations. Treatments were as follows:

C = control = live animals from +5°C

M2 = cold dehydrated animals, cooled to -2°C at a rate of 2°C/week

M7 = cold dehydrated animals, cooled to -7°C at a rate of 2°C/week

H18 = animals taken from -7°C, and left to recover for 18 hours at 4°C with moisture.

Salt 0.9 = animals slowly dehydrated over a saturated solution of sodium nitrate (which gives a constant humidity of 98% RH) to produce animals with a similar water content as the M2 animals

Salt 0.2 = as for Salt 0.9, but the animals were dehydrated to a similar water content to the M7 animals.

6 biological replicates were performed for each condition, as well as some dye swaps.

ORGANISM(S): Megaphorura arctica

SUBMITTER: Michael Thorne 

PROVIDER: E-MEXP-2105 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress