Transcription profiling of Enterococcus faecalis wild type, delta-relA and delta-relAsp (partial deletion) strains
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ABSTRACT: Two mutants of the relA gene of Enterococcus faecalis were constructed, one (delta-relA) carrying a whole deletion of the gene, the other (delta-relAsp) carrying a partial deletion of its 3' extremity. the 3 strains were cultured to mid exponential growth phase in M17 medium, and RNAs were extracted using the RNeasy Midi Kit (QIAGEN).
RNAs were subjected to 2 DNase treatments, and prepared for chips hybridization.
ORGANISM(S): Enterococcus faecalis
SUBMITTER: Vianney PICHEREAU
PROVIDER: E-MEXP-2112 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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