Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Mesenchymal stem cells (MSC) maintained in FCS vs autologous serum analysed at passage 4


ABSTRACT: The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs). To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) with gene expression of MSCs cultured in regular growth medium supplemented with autologous serum (AS) for 3 different donors (i.e. donor2, donor3 and donor4). MSCs were cultured in FCS- or AS-supplemented medium and were analyzed at passage 4.

ORGANISM(S): Homo sapiens

SUBMITTER: Katrine Frønsdal 

PROVIDER: E-MEXP-214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability.

Shahdadfar Aboulghassem A   Frønsdal Katrine K   Haug Terje T   Reinholt Finn P FP   Brinchmann Jan E JE  

Stem cells (Dayton, Ohio) 20050804 9


Human bone marrow mesenchymal stem cells (hMSCs) represent an appealing source of adult stem cells for cell therapy and tissue engineering, as they are easily obtained and expanded while maintaining their multilineage differentiation potential. All current protocols for in vitro culture of hMSCs include fetal bovine serum (FBS) as nutritional supplement. FBS is an undesirable additive to cells that are expanded for therapeutic purposes in humans because the use of FBS carries the risk of transmi  ...[more]

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