Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Brugia Wolbachia elimination


ABSTRACT: Female worms (Brugia malayi) were collected from infected jirds treated with 2.5 mg/ml tetracycline in drinking water for 7, 14, or 21 days to eliminate the worm's endosymbiont, Wolbachia.
Control age matched female worms were recovered from infected jirds given normal water for drinking.
The Filarial Nematode Oligonucleotide Array (version 2) was used in hybridization analyses on cDNA generated from extracted total RNA.
Each microarray was hybridized with a mixture of control and experimental cDNA differentially labeled with Cy3 and Cy5 in a flip-dye experiment.
Gridding and analysis of images were performed using ScanArray v3.0, each spot defined pixel-by-pixel using a modified Mann-Whitney test, and the resulting values processed with Gene-Spring 7.1 software.

ORGANISM(S): Brugia malayi

SUBMITTER: Jay DePasse 

PROVIDER: E-MEXP-2185 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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