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Transcription profiling of Trichoderma reesei QM6a to assess the cellular effects of expression of misfolded CBHI molecules

ABSTRACT: Cultures were grown at 28 oC for 48 h on a shaker at 250 rpm. MG132 (60 ï¾µM, final concentration) in phenylmethylsulfonyl fluoride (PMSF) was added after 46 h or PMSF was added alone to the cultures without MG132. Following sample collection, mycelia were washed with 0.9 % (w/v) NaCl in RNAse-free (diethyl pyrocarbonite [DEPC]-treated) water, frozen in liquid nitrogen and stored at -80 oC.

ORGANISM(S): Trichoderma reesei QM6a

SUBMITTER: Jasmine Grinyer

PROVIDER: E-MEXP-2190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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