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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of fetal livers of UPF2 null mice

ABSTRACT: Deletion of the NMD component UPF2 in fetal liver. Upf2flox/+ females were mated to Upf2flox/+; Alfp-Cre males. Fetal livers were isolated from Upf2+/+; Alfp-Cre (WT) and Upf2flox/flox; AlfpCre (UPF2 null) E16.5 and E18.5 embryos.

ORGANISM(S): Mus musculus

SUBMITTER: Bo Porse

PROVIDER: E-MEXP-2248 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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UPF2 is a critical regulator of liver development, function and regeneration.

Thoren Lina A LA Nørgaard Gitte A GA Weischenfeldt Joachim J Waage Johannes J Jakobsen Janus S JS Damgaard Inge I Bergström Frida C FC Blom Anna M AM Borup Rehannah R Bisgaard Hanne Cathrine HC Porse Bo T BT

PloS one 20100719 7

<h4>Background</h4>Nonsense-mediated mRNA decay (NMD) is a post-transcriptional RNA surveillance process that facilitates the recognition and destruction of mRNAs bearing premature terminations codons (PTCs). Such PTC-containing (PTC+) mRNAs may arise from different processes, including erroneous processing and expression of pseudogenes, but also from more regulated events such as alternative splicing coupled NMD (AS-NMD). Thus, the NMD pathway serves both as a silencer of genomic noise and a re ...[more]

PMID: 20657840

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Transcription profiling of livers of adult UPF2 null mice in response to partial hepatechtomy

Project description:Deletion of the NMD component UPF2 in adult liver and response to partial hepatechtomy. Lethally irradiated Upf2flox/flox and Upf2flox/flox; Mx1Cre animals were transplanted with WT BM at 10-12 weeks of age. 5-6 following transplantion the bone marrow transplanted (BMT) animals were injected 3 times with poly-IC (Days 0, 2, 4), which facilitates Cre-mediated deletion in BMT Upf2flox/flox; Mx1Cre (hitherto UPF2 null) but not in BMT Upf2flox/flox control (hitherto WT) mice. A day 14 mice were subjected to partial hepatechtomy (0 hours) and sacrificed after 36 hours (36 hours)..

2010-06-17 | E-MEXP-2249 | biostudies-arrayexpress

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Upf2 in NMD pathway

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2024-01-26 | PXD040362 | Pride

Upf2 in NMD pathway

2014-09-06 | E-GEOD-58405 | biostudies-arrayexpress

RNA sequencing of blastocyst-stage mouse embryos in the presence or absence of RNA decay activator UPF2

Project description:Purpose: The goal of this study is to compare transcriptome profiles of whole blastocysts in the presence or absence of UPF2, via an approach optimized for low-input samples. Methods: Blastocyst mRNA profiles of wild-type (WT) and Upf2 knockout (Upf2−/−) mice were generated by deep sequencing, using Illumina 2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with DESeq2 and with IsoformSwitchAnalyzeR. Results: We identified transcripts targeted for decay by UPF2 and the NMD pathway in the blastocyst via multiple methods. We found that NMD regulates a cohort of RNAs involved in cell survival.

2022-03-16 | GSE191039 | GEO

Upf2-mediated inhibition of nonsense mediated decay causes memory and social deficits by activating the immune response

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2020-06-29 | GSE81591 | GEO

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