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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Methylation profiling of human type 2 diabetes patients to screen for genes differentially methylated in T2DM MeDIP ARRAY_NGT_vs_T2DM

ABSTRACT: Here, we performed a genome-wide promoter analysis of DNA methylation to screen for genes differentially methylated in T2DM.
We used methylated DNA immunoprecipitation (MeDIP), combined with microarray technology (Keshet et al., 2006; Weber et al., 2005), to discover whether changes in DNA methylation are specific to T2DM. A cohort of normal glucose tolerant (NGT) and Type 2 diabetic (T2DM) male volunteers was studied.

ORGANISM(S): Homo sapiens

DISEASE(S): NGT

SUBMITTER: Romain Barres

PROVIDER: E-MEXP-2253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Non-CpG methylation of the PGC-1alpha promoter through DNMT3B controls mitochondrial density.

Barrès Romain R Osler Megan E ME Yan Jie J Rune Anna A Fritz Tomas T Caidahl Kenneth K Krook Anna A Zierath Juleen R JR

Cell metabolism 20090901 3

Epigenetic modification through DNA methylation is implicated in metabolic disease. Using whole-genome promoter methylation analysis of skeletal muscle from normal glucose-tolerant and type 2 diabetic subjects, we identified cytosine hypermethylation of peroxisome proliferator-activated receptor gamma (PPARgamma) coactivator-1 alpha (PGC-1alpha) in diabetic subjects. Methylation levels were negatively correlated with PGC-1alpha mRNA and mitochondrial DNA (mtDNA). Bisulfite sequencing revealed th ...[more]

PMID: 19723495

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