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Transcription profiling of bovine nucleus pulposus, carticular cartilage and annulus fibrosus cells to further determine expression in normal and degenerate human intervertebral disc

ABSTRACT: The aim of this transcription profiling study was to identify novel genes that could be used to distinguish bovine Nucleus pulposus (NP) cells from articular cartilage (AC) and annulus fibrosus (AF) cells and to further determine their expression in normal and degenerate human intervertebral disc (IVD). This study has identified a number of novel genes that characterise the bovine and human NP and IVD cell phenotypes and allows for discrimination between AC, AF and NP cells.

ORGANISM(S): Bos taurus

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-2291 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiling of bovine intervertebral disc cells: implications for identification of normal and degenerate human intervertebral disc cell phenotypes.

Minogue Ben M BM Richardson Stephen M SM Zeef Leo Ah LA Freemont Anthony J AJ Hoyland Judith A JA

Arthritis research & therapy 20100211 1

<h4>Introduction</h4>Nucleus pulposus (NP) cells have a phenotype similar to articular cartilage (AC) cells. However, the matrix of the NP is clearly different to that of AC suggesting that specific cell phenotypes exist. The aim of this study was to identify novel genes that could be used to distinguish bovine NP cells from AC and annulus fibrosus (AF) cells, and to further determine their expression in normal and degenerate human intervertebral disc (IVD) cells.<h4>Methods</h4>Microarrays were ...[more]

PMID: 20149220

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