Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of Medicago truncatula responses to Colletotrichum species


ABSTRACT: We have previously showed a strong genetic determinant governing resistance of the M. truncatula A17 line to C. trifolii is located in a chromosomal region at the top of chromosome 4 (Ameline-torregrosa et al., 2008). This region also contains the RCT1 gene which has been shown to confer resistance to C. trifolii when transferred to a susceptible alfalfa plants (Zhu et al.), In order to evaluate the role of this region in the response to non-adapted Colletorichum species and to compare this response to those induce by C. trifolii, two M. truncatula near-isogenic lines differing only in this chromosomal region were used for transcript profiling experiments. These two lines were issued from a recombinant-inbred collection obtained from a A17-F83005.5 cross (Cazaux et al., in preparation). Plant inoculations were done on two-week-old entire plants by spraying a conidial suspension of C. trifolii or C. lindemuthianum to avoid possible artifacts which can be observed on detached leaves (Liu et al., 2007). RNA was extracted from leaves collected at 1dpi and 3dpi and transcript profiling was performed using Mt16kOLI1Plus chips (Thompson et al., 2005).

ORGANISM(S): Medicago truncatula

DISEASE(S): 1dpi

SUBMITTER: Bernard Dumas 

PROVIDER: E-MEXP-2349 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Host and nonhost resistance in Medicago-Colletotrichum interactions.

Jaulneau Valérie V   Cazaux Marc M   Wong Sak Hoi Joanne J   Fournier Sylvie S   Esquerré-Tugayé Marie-Thérèse MT   Jacquet Christophe C   Dumas Bernard B  

Molecular plant-microbe interactions : MPMI 20100901 9


Medicago truncatula lines resistant (A17) or susceptible (F83005.5) to the alfalfa pathogen Colletotrichum trifolii were used to compare defense reactions induced upon inoculation with C. trifolii or with the nonadapted pathogens C. lindemuthianum and C. higginsianum. Nonadapted Colletotrichum spp. induced a hypersensitive response (HR)-like reaction similar to the one induced during the host-incompatible interaction. Molecular analyses indicated an induction of PR10 and chalcone synthase genes  ...[more]

Similar Datasets

2009-08-14 | E-MEXP-1758 | biostudies-arrayexpress
2010-01-01 | E-MEXP-1995 | biostudies-arrayexpress
2010-01-01 | E-MEXP-1442 | biostudies-arrayexpress
2009-01-14 | E-MEXP-1441 | biostudies-arrayexpress
2013-07-23 | E-MEXP-3719 | biostudies-arrayexpress
2008-07-31 | E-MEXP-1555 | biostudies-arrayexpress
2012-01-31 | E-MEXP-3485 | biostudies-arrayexpress
2008-05-01 | E-MEXP-1557 | biostudies-arrayexpress
2013-02-12 | E-MEXP-3559 | biostudies-arrayexpress
2009-10-13 | E-MEXP-1854 | biostudies-arrayexpress