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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human memory B cells to plasma cells differentiation time course

ABSTRACT: In vitro model of differentiation of memory B cells into plasmablasts and plasma cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Michel Jourdan

PROVIDER: E-MEXP-2360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization.

Jourdan Michel M Caraux Anouk A De Vos John J Fiol Geneviève G Larroque Marion M Cognot Chantal C Bret Caroline C Duperray Christophe C Hose Dirk D Klein Bernard B

Blood 20091201 25

Human plasma cells (PCs) and their precursors play an essential role in humoral immune response but are rare and difficult to harvest. We report the generation of human syndecan-1(+) and immunoglobulin secreting PCs starting from memory B cells in a 3-step and 10-day (D) culture, including a 6-fold cell amplification. We report the detailed phenotypic and Affymetrix gene expression profiles of these in vitro PCs as well as of intermediate cells (activated B cells and plasmablasts) compared with ...[more]

PMID: 19846886

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