Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

49 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Candida albicans biofilm exposed to amphotericin B, fluconazole or two concentrations of caspofungin

ABSTRACT: 30h of growth biofilms in microfermentors were exposed to caspofungin 0.5 µg/ml, caspofungin 5 µg/ml, fluconazole 80 µg/ml or amphotericin B 8 µg/ml. Control and antifungal-exposed biofilms were recovered at t= 0, 30, 60 and 120 min post exposure to antifungal.

ORGANISM(S): Candida albicans

SUBMITTER: Tristan Rossignol

PROVIDER: E-MEXP-2436 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Interaction of Candida albicans biofilms with antifungals: transcriptional response and binding of antifungals to beta-glucans.

Vediyappan Govindsamy G Rossignol Tristan T d'Enfert Christophe C

Antimicrobial agents and chemotherapy 20100301 5

Candida albicans can form biofilms that exhibit elevated intrinsic resistance to various antifungal agents, in particular azoles and polyenes. The molecular mechanisms involved in the antifungal resistance of biofilms remain poorly understood. We have used transcript profiling to explore the early transcriptional responses of mature C. albicans biofilms exposed to various antifungal agents. Mature C. albicans biofilms grown under continuous flow were exposed for as long as 2 h to concentrations ...[more]

PMID: 20194705

Similar Datasets

Transcription profiling by array of yeast haploid Sigma1278b strains to detect Yak1-regulated genes

Project description:We determined and classified Yak1-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of different haploid MATa yeast strains of the following genotypes:YAK1 (468=YHUM0468), yak1 (1313=YHUM1313), strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 (1313Yhc) and strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 K398R (1313KDhc). All strains were grown in duplicate in YNB medium supplemented with tryptophan at 30 degrees Celsius to an optical density of 1.0 before extraction of total RNA and transcriptional profiling.

2011-08-23 | E-MEXP-2885 | biostudies-arrayexpress

Transcription profiling by array of yeast haploid Sigma1278b wildtype strain and whi3 deletion strain

Project description:We determined and classified Whi3-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of two different haploid MATa yeast strains of the following genotypes: WHI3 strain (468=YHUM0468) and whi3 strain (1105=YHUM1105).Both starins were grown in duplicate in YNB medium with tryptophan at 30°C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling

2011-07-08 | E-MEXP-2810 | biostudies-arrayexpress

Schladebeck_Whi3_Ploidy

Project description:We analyzed and classified Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of two different haploid MATa and one diploid MATa/a yeast strains of the following genotypes: WHI3 strain (SS_YHUM468=YHUM0468), whi3 strain (SS_ySS137=YHUM1920) and whi3-delta/whi3-delta strain (SS_ySS137dipl=YHUM2152). All three strains were grown in duplicate in YNB medium supplemented with tryptophan and uracil at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling

2013-06-19 | E-MEXP-3610 | biostudies-arrayexpress

Transcription profiling of Burkholderia cenocepacia J2315 sessile cells to treatment with high doses of hydrogen peroxide, sodium hypochlorite and chlorhexidine

Project description:In order to unravel the molecular mechanisms involved in the resistance of biofilm-grown Burkholderia cenocepacia cells against high concentrations of disinfectants, the present study focussed on the transcriptional response in sessile B. cenocepacia J2315 cells following exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.

2010-03-02 | E-MEXP-2502 | biostudies-arrayexpress

Transcription profiling of Candida albicans treated with supernatants from four strains of Pseudomonas aeruginosa

Project description:Effects of supernatants from four strains of Pseudomonas aeruginosa on global gene expression in Candida albicans

2008-11-12 | E-MEXP-1614 | biostudies-arrayexpress

HpHap4 proteins in the context of Sc delta hap4 mutant

Project description:The effect of overexpression HpHAP4-A, HpHAP4-B and ScHAP4 in Sc delta hap4 genetic background.

2011-06-19 | E-MEXP-3173 | biostudies-arrayexpress

Transcription profiling of Streptococcus gordonii wildtype and Brf- deletion and insertion mutants

Project description:RNA was isolated from early stationary phase S. gordonii V288(OD620 nm ~ 1.8).Bacteria were grown in chemically defined synthetic media (FMC) ( K2HPO4, 300 µg ml-1; KH2PO4 440 µg ml-1; Na2HPO4, 3.15 mg ml-1; NaH2PO4, 2.05 mg ml-1; (NH4)2SO4 0.6 mg ml-1; sodium citrate, 225 µg ml-1; riboflavin, 0.4 µg ml-1; biotin, 0.01 µg ml-1; folic acid, 0.1 µg ml-1; pantothenate, 0.8 µg ml-1; p-aminobenzoic acid 0.1 µg ml-1; thiamine 0.4 µg ml-1; nicotinamide 2.0 µg ml-1; pyridoxamine 0.8 µg ml-1; D-glucose 20 mg ml-1; sodium acetate 6 mg ml-1; adenine 35 µg ml-1; guanine µg ml-1; uracil µg ml-1; MgSO4, 200 µg ml-1; NaCl, 10 µg ml-1; FeSO4, 10 µg ml-1; MnSO4 10 µg ml-1; glutamine 5 µg ml-1; L-glutamic acid, 300 µg ml-1; L-valine, 100 µg ml-1; 110 µg ml-1 of L-lysine, L-asparate, L-isoleucine, L-methionine, L-serine, L-phenylalanine and 200 µg ml-1 of L-alanine, L-cystine, L-histidine, glycine, L-hydroxyproline, L-proline, L-tryptophan and L-tyrosine, pH 6.5) (Terleckyj & Shockman, 1975; Terleckyj et al., 1975) at 37°C in 5% CO2.

2008-08-01 | E-MEXP-1712 | biostudies-arrayexpress

Transcription profiling by array of human thalassemic and normal CD34 positive cells before and after cytokine activation

Project description:To understand the impact of cytokines stimulation on global gene expression profile of CD34 positive cells from patients, we extracted RNA from cells before and after in vitro stimulation to determine transcriptional profile on the Affimetrix HG-U133 2.0 Plus platform. The expression profiling data from a total of 20 thalassemic and 22 normal samples, including adult and pediatric subjects, were analyzed.

2010-08-01 | E-MEXP-2757 | biostudies-arrayexpress

Transcription profiling of yeast sum1 delta strain at three time points after the start of meiosis

Project description:Expression profiling of sum1 delta strain at three time points after the start of meiosis

2010-04-20 | E-MEXP-2154 | biostudies-arrayexpress

Transcription profiling of yeast during meiosis in a strain with Ndt80 induced with estradiol

Project description:Expression profile of meiosis in strain with Ndt80 inducible by addition of Estradiol (as in Carlile and Amon 2008), in three time points from addition of Estradiol.

2010-04-20 | E-MEXP-2155 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data