Transcription profiling of mouse erythroleukaemic cell line after DSMO-induced differentiation

ABSTRACT: Mouse ErythroLeukaemic (MEL) cell lines were maintained in DMEM
supplemented with 10% fetal calf serum and penicillin/streptomycin. MEL
cells in the log phase of growth (0.5 to 1 millions cells / mL) were
induced to differentiate with 2% dimethylsulfoxide (DMSO) for four days.
Total RNA was isolated in triplicate from non-induced and DMSO-induced MEL
cells using RNeasy Mini Kit (Qiagen). Purity and quality of isolated RNA
were assessed by RNA 6000 Nano assay on a 2100 Bioanalyzer (Agilent
Technologies, Santa 6 Clara, CA). All samples showed a RIN>8 and were all
used for subsequent labeling. RNA (300 ng) was used for production of
end-labelled biotinylated ssDNA. Labelled ssDNA was hybridized to the
GeneChip Mouse Gene 1.0 ST array oligonucleotide microarray (A

ORGANISM(S): Mus musculus

SUBMITTER: Jan Christian Bryne 

PROVIDER: E-MEXP-2454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress