Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Microglia treatment with C6 glioma-conditioned medium or with LPS

ABSTRACT: Several independent microglia preparations performed on different days were plated onto wells of 24-well plates, and either left untreated (control), treated with the C6 glioma-conditioned medium, or treated with LPS.
For each culture condition, a number of RNA samples were prepared, with each sample then separately labeled and hybridized. These RNA samples were prepared from non-overlapping sets of wells from at least two microglia preparations, so they constitute biological replicates.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Michal Dabrowski

PROVIDER: E-MEXP-2466 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Early steps of microglial activation are directly affected by neuroprotectant FK506 in both in vitro inflammation and in rat model of stroke.

Zawadzka Malgorzata M Dabrowski Michal M Gozdz Agata A Szadujkis Barbara B Sliwa Marcin M Lipko Maciej M Kaminska Bozena B

Journal of molecular medicine (Berlin, Germany) 20120718 12

Neuroprotective and/or neuroregenerative activity of FK506, its derivatives, and to a lesser extent cyclosporin A (CsA) in animal models of neurodegenerative diseases of different etiology have been reported. Here, we verified a hypothesis that the most likely mechanism of their neuroprotective action is inhibition of the early steps of inflammatory activation of microglia by interference with mitogen-activated protein kinase (MAPK) signaling. The effect of immunosuppressants on lipopolysacchari ...[more]

PMID: 22806180

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Dataset Information

Microglia treatment with C6 glioma-conditioned medium or with LPS

Publications

Early steps of microglial activation are directly affected by neuroprotectant FK506 in both in vitro inflammation and in rat model of stroke.

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