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Transcriptional control of a plant stem cell niche

ABSTRACT: We used a systems approach to identify target genes and genome wide chromatin binding preferences of the transcription factor WUSCHEL (WUS) in Arabidopsis thaliana. First, we recorded changes in the transcriptome after genetically perturbing the regulatory system of the stem cell niche at various developmental stages by loss-of-function and inducible over-expression alleles of WUS and its antagonist CLV3. Then, identified direct WUS target genes by chromatin immuno-precipitation (ChIP.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Wolfgang Busch

PROVIDER: E-MEXP-2499 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional control of a plant stem cell niche.

Busch Wolfgang W Miotk Andrej A Ariel Federico D FD Zhao Zhong Z Forner Joachim J Daum Gabor G Suzaki Takuya T Schuster Christoph C Schultheiss Sebastian J SJ Leibfried Andrea A Haubeiss Silke S Ha Nati N Chan Raquel L RL Lohmann Jan U JU

Developmental cell 20100501 5

Despite the independent evolution of multicellularity in plants and animals, the basic organization of their stem cell niches is remarkably similar. Here, we report the genome-wide regulatory potential of WUSCHEL, the key transcription factor for stem cell maintenance in the shoot apical meristem of the reference plant Arabidopsis thaliana. WUSCHEL acts by directly binding to at least two distinct DNA motifs in more than 100 target promoters and preferentially affects the expression of genes wit ...[more]

PMID: 20493817

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