Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of mouse liver from control, pregnant, cholate fed mice and Frx null mice

ABSTRACT: Liver gene expression in control, pregnant (18dpc), 0.5% cholate fed (30-days), Fxr-/-

ORGANISM(S): Mus musculus

SUBMITTER: Bryn Owen

PROVIDER: E-MEXP-2505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Raised hepatic bile acid concentrations during pregnancy in mice are associated with reduced farnesoid X receptor function.

Milona Alexandra A Owen Bryn M BM Cobbold Jeremy F L JF Willemsen Ellen C L EC Cox Isobel J IJ Boudjelal Mohamed M Cairns William W Schoonjans Kristina K Taylor-Robinson Simon D SD Klomp Leo W J LW Parker Malcolm G MG White Roger R van Mil Saskia W C SW Williamson Catherine C

Hepatology (Baltimore, Md.) 20101001 4

<h4>Unlabelled</h4>Pregnancy alters bile acid homeostasis and can unmask cholestatic disease in genetically predisposed but otherwise asymptomatic individuals. In this report, we show that normal pregnant mice have raised hepatic bile acid levels in the presence of procholestatic gene expression. The nuclear receptor farnesoid X receptor (FXR) regulates the transcription of the majority of these genes, and we show that both ablation and activation of Fxr prevent the accumulation of hepatic bile ...[more]

PMID: 20842631

Similar Datasets

Pregnant Sepsis(Mice)

Project description:Pregnant Sepsis(Mice)

| PRJEB48154 | ENA

Gene expression analysis of FXR null, SHP null, and double FXR/SHP null mice

Project description:Bile acid (BA) homeostasis is maintained through a feedback loop operated by the nuclear hormone receptors FXR and SHP. Here we show that contrary to the current models placing FXR upstream of SHP in a linear regulatory pathway, the phenotypic consequence of the combined loss of both receptors is much more severe than the relatively modest impact of the loss of either Fxr or Shp alone. This is highlighted by the dramatic elevation of hepatic and serum BA levels in the double knockout (DKO) mice as early as three weeks of age coupled with a commensurate increase in Cyp7A1 expression and alterations in BA homeostatic genes. In addition, we find several genes necessary for C21 steroid biosynthetic pathway as novel targets for FXR and SHP. The elevated BAs result in severe hepato-pathology but the DKO mice surprisingly do not develop complete liver failure and live for over a year. Their survival is accompanied by an adaptive proliferation of the resident liver progenitor cell population, known as oval cells. Overall, these data demonstrate that FXR and SHP function coordinately to maintain BA homeostasis, and identify DKO mice as a novel genetic model for juvenile cholestatic disorders and for oval cell activation. Liver samples collected from FXR-/-, SHP-/-, and FXR-/-/SHP-/- animals at 3 or 5 weeks were hybridized to Illumina mouse REF-8 v1.1 arrays in duplicate.

2010-12-10 | E-GEOD-20599 | biostudies-arrayexpress

The role of hepatic XBP1 in FXR null mice

Project description:X-box binding protein 1 (XBP1) is a key component of the unfolded protein response that is activated in response to ER stress. Farnesoid X receptor (FXR) null (Fxr-/-) mice have elevated bile acid levels and progressive liver injury. Hepatic XBP1 pathway is activated in older Fxr-/- mice. This project is to investigate the role of liver XBP1 in Fxr-/- mice.

2022-09-19 | GSE201902 | GEO

Gene expression analysis of FXR null, SHP null, and double FXR/SHP null mice

2010-12-10 | GSE20599 | GEO

Transcriptional profiles of biliary epithelial cells from non-pregnant mice and pregnant mice at dpc8

Project description:We report transcriptional profiles of liver biliary epithelial cells (BECs) from non-pregnant mice and pregnant mice. By RNA-sequencing of FACS-isolated BECs, we revealed that genes related to tissue morphogenesis and actin cytoskeleton were upregulated on dpc8.

2023-04-01 | GSE189873 | GEO

Hepatic Farnesoid X-Receptor Isoforms α2 and α4 Differentially Modulate Bile Salt and Lipoprotein Metabolism in Mice

Project description:The nuclear receptor FXR acts as an intracellular bile salt sensor that regulates synthesis and transport of bile salts within their enterohepatic circulation. In addition, FXR is involved in control of a variety of crucial metabolic pathways. Four FXR splice variants are known, i.e. FXRα1-4. Although these isoforms show differences in spatial and temporal expression patterns as well as in transcriptional activity, the physiological relevance hereof has remained elusive. We have evaluated specific roles of hepatic FXRα2 and FXRα4 by stably expressing these isoforms using liver-specific self-complementary adeno-associated viral vectors in total body FXR knock-out mice. The hepatic gene expression profile of the FXR knock-out mice was largely normalized by both isoforms. Yet, differential effects were also apparent; FXRα2 was more effective in reducing elevated HDL levels and transrepressed hepatic expression of Cyp8B1, the regulator of cholate synthesis. The latter coincided with a switch in hydrophobicity of the bile salt pool. Furthermore, FXRα2-transduction caused an increased neutral sterol excretion compared to FXRα4 without affecting intestinal cholesterol absorption. Our data show, for the first time, that hepatic FXRα2 and FXRα4 differentially modulate bile salt and lipoprotein metabolism in mice.

2014-11-11 | GSE51805 | GEO

Effect of MCMV infection on gene expression in ovaries of pregnant and non-pregnant mice

Project description:Affymetrix Gene ST 1.0 arrays were performed on total RNA obtained from ovaries of 5 female mice (pregnant or non-pregnant; mock or MCMV infection). Mice were infected 9.5 days post conception with 2x10e05 PFU MCMV i.v.. RNA was prepared at 5 days post infection (14.5 days post conception).

2016-09-03 | GSE51845 | GEO

Transcriptional profiles of liver in control and p21-AAV8 administered non-pregnant mice and pregnant mice at dpc8 and dpc16.

Project description:We report transcriptional profiles of liver from control and p21-AAV8 administered no-pregnant mice and pregnant mice at dpc8 and dpc16. We revealed that genes related to carbohydrate transport and regulators of glucose and glycogen metabolism were down regulated in AAV-p21 administered liver at dpc16.

2023-12-13 | GSE181875 | GEO

Expression data from pancreatic islets of prolactin receptor null pregnant mice

Project description:Expression data from pancreatic islets of prolactin receptor null pregnant mice

| PRJNA515905 | ENA

Transcriptional profiles of liver lobular zones from non-pregnant mice and pregnant mice at dpc8 and dpc16.

Project description:We report transcriptional profiles of each liver lobular zone from non-pregnant mice and pregnant mice at dpc8 and dpc16. By RNA-sequencing of LCM-isolated each zonal cells, we revealed that genes related to actin cytoskeleton, extracellular matrix organization and vascularization were enriched in the PA (zone2) and CV (zone3) zones at dpc8 and cell division related genes were enriched in the PV (zone1) zone at dpc8. At dpc16, genes related to metabolism, catabolism and xenobiotic process were enriched in all zones.

2023-12-13 | GSE181874 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data