Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

38 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of Treponema denticola in response to environmental stresses

ABSTRACT: Whole genome expression profiles in response to different suspected stresses including heat, hypo-tonic conditions, oxygen and blood exposure were examined.

ORGANISM(S): Treponema denticola

SUBMITTER: Ian McHardy

PROVIDER: E-MEXP-2656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Td multispecies interactions

Project description:We utilized T. denticola as a model red complex organism to help determine whether red complex organisms can respond to one another upon cell-cell contact in mixed culture and the types of genes involved in such responses. We extracted RNA from combinations of T. denticola with either other P. intermedia, S. sanguinis, S. gordonii, F. nucleatum, P. gingivalis, or T. forsythia that had been centrifuged to induce cell contact, and identified the numbers and types of genes that were differentially regulated using microarrays.

2013-08-10 | E-MEXP-3059 | biostudies-arrayexpress

Fn multispecies biofilm experiment

Project description:This study was conducted to identify whether F. nucleatum has species or group specific transcriptional responses by analyzing dual species biofilms containing either early or late colonizers and utilizing microarrays to identify altered gene expression of F. nucleatum.

2011-11-30 | E-MEXP-3058 | biostudies-arrayexpress

20% (w/v) Glucose osmotic stress (YP20) vs 1M Sorbitol osmotic stress (YPS)

Project description:Transcriptional profiling of yeast Saccharomyces serevisiae cells exposed to stress (30 min) compared to no stress conditions (YPD) Two stress condition experiments, YP20 and YPS, each one compared to no-stress conditions (YPD). Two replicates of each condition

2015-01-01 | E-GEOD-59659 | biostudies-arrayexpress

Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni

Project description:The body’s defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host immune system. The most common treatment for schistosomiasis is the anti-helmenthic drug praziquantel. Its effectiveness, however, is limited due to its inability to kill schistosomes 2 - 4 weeks post-infection. Clearly there is a need for new anti-schistosomal drugs. We hypothesize that gene products expressed as part of a protective response against heat and/or oxidative stress are potential therapeutic targets for future drug development. Using a 12,166 element oligonucleotide microarray to characterize Schistosoma mansoni genes induced by heat and oxidative stress we found that 1,878 Schistosoma mansoni elements were significantly induced by heat stress. These included previously reported heat-shock genes expressing homologs of HSP40, HSP70 and HSP86. One thousand and one elements were induced by oxidative stress including those expressing homologs of superoxide dismutase, glutathione peroxidase and aldehyde dehydrogenase. Seventy-two elements were common to both stressors that could potentially be exploited in the development of novel anti-schistosomal therapeutics. Keywords: Stress response, time course Eight samples performed in duplicate for each temperature and oxidative stresses at time points 0, 30, 60, and 240 min over a common reference sample for each stress

2008-08-25 | E-GEOD-11706 | biostudies-arrayexpress

Ferrous iron sensing in Pseudomonas aeruginosa

Project description:To determine whether P. aeruginosa strain PA14 exhibits a specific transcriptional response to extracellular Fe(II), a microarray experiment was performed using Affymetrix GeneChips. The transcriptional response to Fe(II) or Fe(III) shock was measured and compared to a no-Fe control.

2012-02-29 | E-MEXP-3459 | biostudies-arrayexpress

LVS oxidative stress

Project description:Francisella tularensis LVS was grown to mid log phase and culture split into two. One culture served as control and other added H2O2 to final concentration of 0.03%. After 20 min at 37 C, RNA was isolated from both cultures

2012-02-01 | E-MEXP-3398 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on complex liquid medium

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in complex liquid broth.

2009-05-01 | E-MEXP-1891 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on defined liquid medium (Chamberlains medium)

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in defined liquid medium (Chamberlain's medium)

2009-05-01 | E-MEXP-1892 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS carrying plasmid pFNLTP6-gro or pFNLTP-gro-0851 (expressing rpoH) grown to mid exponential phase

Project description:F. tularensis LVS carrying plasmid pFNLTP6-gro or pFNLTP-gro-0851 (expressing rpoH) were grown to mid exponential phase in Schaedler + vitamin K3 medium and RNA isolated.

2009-07-01 | E-MEXP-2048 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS after heat shock

Project description:Effect of temperature upshift at 44°C for 30 minutes on transcription in Francisella tularensis LVS

2009-07-01 | E-MEXP-2037 | biostudies-arrayexpress