Transcription profiling by array of human universal RNA spiked with eight arifical transcripts at different copy numbers

ABSTRACT: Examine the dose response characteristics of a set of eight artificial transcripts (IVT) when spiked into a Universal Human RNA background at different copy numbers. The experiment was designed to
(i) evaluate the technical reproducibility of array data generated on the Agilent Whole Human 44K array with use of a set of external RNA controls
(ii) employ two panels of the same external RNA controls, which differed in copy number between panels, in order to simulate normal and disease states. We employed 8 different pools which were comprised of 8 different external RNA standards in a total human reference RNA background. Each different standard was present at a different copy number within any single pool (see file: E-MEXP-2670.additional.zip). Each control was present at a different concentration in each of the 8 pools. Standard microarray analysis was performed on the data and the differences in abundance between external RNA controls determined in terms of fold change differences.

ORGANISM(S): Homo sapiens

SUBMITTER: timothy wilkes 

PROVIDER: E-MEXP-2670 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress