Transcription profiling by array of human NKX2.1GFP/w spin embryoid bodies differentiated and then FACS sorted based on FORSE-1, NKX2.1-GFP and PDGFR expression
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ABSTRACT: The gene targeted NKX2.1GFP/w hESC line was differentiated as spin EBs in BPEL medium supplemented with FGF2 and retinoic acid to generate NKX2.1-GFP+ neural cells. NKX2.1-GFP+ cells were FACS sorted and further differentiated in serum free BEL medium supplemented with FGF2 (20 ng/ml) and EGF1 (20 ng/ml) on laminin-coated flasks. After ~ 90 days in culture, cells were FACS sorted based on FORSE-1 and NKX2.1-GFP expression, with the FORSE-1-GFP- fraction further fractionated on the basis of PDGFR? expression. Sorted fractions were subjected to Illumina microarray processing.
ORGANISM(S): Homo sapiens
SUBMITTER: Qing Yu
PROVIDER: E-MEXP-2808 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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