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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-chip by array of yeast JTY1, SHY546, BY4743, and Bur1 strains to investigate genome-wide patterns of RNA Polymerase II CTD Phosphorylation

ABSTRACT: Chromatin Immunoprecipitation in JTY1, SHY546, BY4743, and Bur1as strains using antibodies against RNA Pol II (RPB3) and its 3 phosphorylation states Ser2-P, Ser5-P, and Ser7-P, with and without inhibition with small molecule inhibitors.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Josh Tietjen

PROVIDER: E-MEXP-2850 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chemical-genomic dissection of the CTD code.

Tietjen Joshua R JR Zhang David W DW Rodríguez-Molina Juan B JB White Brent E BE Akhtar Md Sohail MS Heidemann Martin M Li Xin X Chapman Rob D RD Shokat Kevan K Keles Sündüz S Eick Dirk D Ansari Aseem Z AZ

Nature structural & molecular biology 20100829 9

Sequential modifications of the RNA polymerase II (Pol II) C-terminal domain (CTD) coordinate the stage-specific association and release of cellular machines during transcription. Here we examine the genome-wide distributions of the 'early' (phospho-Ser5 (Ser5-P)), 'mid' (Ser7-P) and 'late' (Ser2-P) CTD marks. We identify gene class-specific patterns and find widespread co-occurrence of the CTD marks. Contrary to its role in 3'-processing of noncoding RNA, the Ser7-P marks are placed early and r ...[more]

PMID: 20802488

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