Project description:Both wild-type and mutant A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in GM plates for 3 weeks under a 16 h light/8 h dark regimen (40 ± 10 umol photons/m2/s) at 22°C. Dehydration treatment: The 3-week-old plants were grown for 2 h without watering.
Project description:Both wild-type and SPY-3 OX A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in GM plates for 3 weeks under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22M-0C. Dehydration treatment: The 3-week-old plants were grown for 2 h without watering.
Project description:We compared the expression profiles of amiG7 mutant with those of the wild type plants using an Agilent Arabidopsis 3 Oligo Microarray.
Project description:Three-week-old DEX-AZF1, DEX-AZF2, and vector control plants were treated 10 µM DEX solution for 24 h. Two-week-old ProAZF2:AZF2 and vector control plants were treated with water as control or 200 mM NaCl for 5 h.
Project description:Arabidopsis thaliana (L.) Heynh. ecotype Columbia was used in this study. Seeds were sterilized and stratified at 4 C for 2 days in the dark, sown, and grown on germination media (GM) agar plates in a growth chamber with 16 hours of fluorescent light at 40M-110 ?mol m-2 sec-1, 22 C, and 70% relative humidity.<br><br> The T-DNA insertion line for GRF7 was obtained from the ABRC (Stock No. CS878963;? Sessions et al., 2002). The T-DNA insertion was confirmed by amplifying the LB-flanking region in genomic DNA of individual candidate lines.
Project description:ZmDREB2A is a DREB2-type transcription factor cloned from maize, whose transcript was upregulated by drought, high salt, low temperature and heat stresses. The ZmDREB2A gene possesses two kinds of transcription forms by alternative splicing. Only the functional form was studied to be highly induced by stresses. Transgenic plants overexpressing ZmDREB2A (35S:ZmDREB2A) showed dwarfism and enhanced drought stress tolerance. Microarray analysis of two independent transgenic plants revealed that in addition to genes encoding LEA proteins, some genes related to heat shock and detoxification were also upregulated. Experiments on termotolerance tests of these transgenic plants showed overexpressing ZmDREB2A gene also improved plant tolerance to heat stress.
Project description:ATHK1 is a histidine kinase responsive to drought stress.In this experiment, gene expression profile of athk1 knockdown mutant was compared with that of the wild_type under unstressed condition.