Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of barley roots during adaptation to abiotic stress conditions

ABSTRACT: Changes in gene expression during adaptation to abiotic stress conditions in the barley roots.

ORGANISM(S): Hordeum vulgare

SUBMITTER: Nils Rostoks

PROVIDER: E-MEXP-301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Global landscape of a co-expressed gene network in barley and its application to gene discovery in Triticeae crops.

Mochida Keiichi K Uehara-Yamaguchi Yukiko Y Yoshida Takuhiro T Sakurai Tetsuya T Shinozaki Kazuo K

Plant & cell physiology 20110324 5

Accumulated transcriptome data can be used to investigate regulatory networks of genes involved in various biological systems. Co-expression analysis data sets generated from comprehensively collected transcriptome data sets now represent efficient resources that are capable of facilitating the discovery of genes with closely correlated expression patterns. In order to construct a co-expression network for barley, we analyzed 45 publicly available experimental series, which are composed of 1,347 ...[more]

PMID: 21441235

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Project description:Background: Lysine crotonylation (Kcr), as a novel evolutionarily conserved type of PTM, is ubiquitous and essential in cell biology. However, its functions in tea plant, an important beverage crop, are largely unknown. Our study firstly attempted to describe Kcr proteins in tea leaves under NH4+ deficiency/resupply, and provided significant insights into exploring the physiological role of Kcr in plants for N utilization. Results: We performed the global analysis of crotonylome in tea plants under NH4+ deficiency/resupply using high-resolution LC-MS/MS coupled with highly sensitive immune-antibody. A total of 2288 kcr sites on 971 proteins were identified, of which contained in 15 types of Kcr motifs. Most of Kcr proteins were located in chloroplast and cytoplasm. 120 and 151 Kcr proteins were significantly changed at 3 hours and 3days of NH4+ resupply, respectively. Bioinformatics analysis showed that differentially expressed Kcr proteins participated in diverse biological processes such as photosynthesis, carbon fixation and amino acid metabolism, suggesting Kcr plays important roles in these processes. Interestingly, a large number of enzymes were crotonylated, and the activity and Kcr level of these enzymes changed significantly after NH4+ resupply, indicating a potential function of Kcr in the regulation of enzyme activities. Moreover, the protein-protein interaction analysis revealed that the diverse interactions of identified Kcr proteins mainly involved in photosynthesis, carbon fixation, amino acid metabolism and ribosome. Conclusions: The results suggested that lysine crotonylated proteins might play regulating roles in metabolic process in tea leaves under NH4+ deficiency/resupply. The critical regulatory roles mainly involved in diverse aspects of primary metabolic processes, especially in photosynthesis, carbon fixation and amino acid metabolism. The provided data may serve as important resources for exploring the physiological, biochemical, and genetic role of lysine crotonylation in tea plants.

Dataset Information

Transcription profiling of barley roots during adaptation to abiotic stress conditions

Publications

Global landscape of a co-expressed gene network in barley and its application to gene discovery in Triticeae crops.

Similar Datasets

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