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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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CCCB - Genomic variation

ABSTRACT: Antibiotic resistance in Streptococcus pneumoniae is often the result of horizontal gene transfer events involving closely related streptococcal species. Laboratory experiments confirmed that S. mitis DNA functions as donor in transformation experiments, using the laboratory strain S. pneumoniae R6 as recipient and chromosomal DNA of a high level penicillin resistant S. mitis B6 strain. After four transformation steps, alterations in five penicillin-binding proteins (PBP) were observed, and sequence analysis confirmed recombination events in the corresponding PBP genes. In order to detect regions where recombination with S. mitis DNA has occurred we analyzed the S. pneumoniae transformants by microarray analyses, using oligonucleotide microarrays designed for the S. pneumoniae genome and the S. mitis B6 genome as well.

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Martin Rieger

PROVIDER: E-MEXP-3170 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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