Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression of Drosophila melanogaster in response to multiple cold exposures

ABSTRACT: In the field, insects suffer multiple cold exposures during winter. When exposed to repeated low temperatures, Drosophila melanogaster females showed an increase in survival, but a reduction in reproduction. In this study, the microarrays were used to analyze the gene expression of female D. melanogaster after multiple, single sustained (or single prolonged) and single short cold treatments, which exposed the flies at 0 M-0C for repeated 2 h, single 10 h and single 2 h respectively. Candidate genes that were involved in 6 h recovery from different types of cold exposures were identified. After repeated cold exposures, candidates particularly included genes involved in muscle protein and muscle activity. Stress-related genes, Turandot A, Turandot C, and Turandot M were up-regulated in response to multiple cold exposures, and improve the cold survival in female D. melanogaster. This work also suggested a strong relationship between cold exposure and the immune system. I suggest that in fruit flies, chilling injuries after cold exposure may induce immune responses and contribute to recovery from cold.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jian Zhang

PROVIDER: E-MEXP-3194 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Divergent transcriptomic responses to repeated and single cold exposures in Drosophila melanogaster.

Zhang Jian J Marshall Katie E KE Westwood J Timothy JT Clark Melody S MS Sinclair Brent J BJ

The Journal of experimental biology 20111201 Pt 23

Insects in the field are exposed to multiple bouts of cold, and there is increasing evidence that the fitness consequences of repeated cold exposure differ from the impacts of a single cold exposure. We tested the hypothesis that different kinds of cold exposure (in this case, single short, prolonged and repeated cold exposure) would result in differential gene expression. We exposed 3 day old adult female wild-type Drosophila melanogaster (Diptera: Drosophilidae) to -0.5°C for a single 2 h expo ...[more]

PMID: 22071194

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Project description:We combined heritability analysis of larval development rate with a global expression analysis of this phenotype to investigate genotype by environment interactions across three ecologically relevant temperatures in the Glanville fritillary butterfly (Melitaea cinxia). We focused upon the development of final instar caterpillars which is greatly affected by temperature, and during this stage the caterpillars build up most of the resources for adult life. Second generation, lab reared larvae, initially collected from the M-CM-^Eland metapopulation, were reared in standard lab condition until 6th larval instar. At the beginning of the final (7th) instar stage the larvae were separated into of three temperature conditions: Cold treatment (temperature profile: 8M-BM-0C 18:00-9:59, 14M-BM-0C 10:00-11:59, 20M-BM-0C 12:00-15:59 and 14M-BM-0C 16:00-17:59) Standard treatment (temperature profile: 15M-BM-0C 17:00-6:59, 18M-BM-0C 7:00-8:59, 22M-BM-0C 9:00-10:59 and 26M-BM-0C 11:00-16:59) Hot treatment (temperature profile: 8M-BM-0C 20:00-7:59, 15M-BM-0C 8:00-9:59, 35M-BM-0C 10:00-17:59 and 15M-BM-0C 18:00-19:59) The temperature profiles mimic the diurnal thermal variation of the natural habitat (M-CM-^Eland islands) of samples. Cold treatment mimics a cool and cloudy summer, Standard represents an average temperature profile in the M-CM-^Eland islands and Hot treatment mimics an exceptionally hot and sunny summer, with cold night-time temperatures. The experiment contained several larval families (full-sib) of which three were selected for gene expression analysis. Samples were snap-frozen in liquid nitrogen during mid-development (after 6, 5 and 4 days, for Cold, Standard and Hot respectively). Additional larvae from the same treatments were assayed for survival and growth. Gene expression was analyzed using a mixed model approach to identify genes with potential heritable expression variation (variation among families), genes with plastic expression responses (treatment induced changes) and genes with treatment dependent expression that varies among families (family by treatment interactions). Full-sib larvae from three families (N170, N74 and O171) were exposed to three temperature treatments (Cold, Standard and Hot) during final (7th) instar stage. A total of 35 samples were used to analyze family, treatment and family by treatment interactions in gene expression, using a mixed model approach. This included 3 biological replicates in Cold and Hot treatments from each family and 5-6 biological replicates in Standard (5 in O171) per family. Techinal replicates from each family and additional techical replicates in family O171 (including dye swap replicates) were used to assess robustness of the findings.

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Dataset Information

Gene expression of Drosophila melanogaster in response to multiple cold exposures

Publications

Divergent transcriptomic responses to repeated and single cold exposures in Drosophila melanogaster.

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