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SF-1 and RNF31 knockdown and forskolin treatment

ABSTRACT: The human adrenocortical carcinoma cell line NCI-H295R treated with siRNA targeting SF-1 or RNF31 with luciferase-targeting siRNA as control. Combined with forskolin treatment.

ORGANISM(S): Homo sapiens

SUBMITTER: Philip Jonsson

PROVIDER: E-MEXP-3259 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Knockdown of SF-1 and RNF31 affects components of steroidogenesis, TGFβ, and Wnt/β-catenin signaling in adrenocortical carcinoma cells.

Ehrlund Anna A Jonsson Philip P Vedin Lise-Lotte LL Williams Cecilia C Gustafsson Jan-Åke JÅ Treuter Eckardt E

PloS one 20120309 3

The orphan nuclear receptor Steroidogenic Factor-1 (SF-1, NR5A1) is a critical regulator of development and homeostasis of the adrenal cortex and gonads. We recently showed that a complex containing E3 ubiquitin ligase RNF31 and the known SF-1 corepressor DAX-1 (NR0B1) interacts with SF-1 on target promoters and represses transcription of steroidogenic acute regulatory protein (StAR) and aromatase (CYP19) genes. To further evaluate the role of SF-1 in the adrenal cortex and the involvement of RN ...[more]

PMID: 22427816

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