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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effect of fetal HDL on gene expression in human placental endothelial cells

ABSTRACT: The aim of this study was to identify HDL and apoE-regulated genes in human placental endothelial cells (HPEC), which are exposed to fetal HDL. HPECs extracted from 5 human placentas were cultivated and treated for 16 h with 15ug/ml fetal HDL, 15ug/ml reconstituted HDL (rHDL), or endothelial basal medium (EBM) as vehicle control. Gene expression analysis from these 3 conditions (5 biological replicates) using 15 Applied Biosystems Human Whole Genome Survey V2.0 Microarrays was perfomed and significantly differentially expressed genes between two different groups (HDL vs control or rHDL vs control) were identified.

ORGANISM(S): Homo sapiens

SUBMITTER: Hubert Hackl

PROVIDER: E-MEXP-3299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fetal HDL/apoE: a novel regulator of gene expression in human placental endothelial cells.

Augsten Manuela M Hackl Hubert H Ebner Birgit B Chemelli Angela A Glatter Otto O Marsche Gunther G Lang Uwe U Desoye Gernot G Wadsack Christian C

Physiological genomics 20110927 22

Maternal lipoproteins have been studied extensively in human pregnancies, but little is known about the role of fetal lipoproteins. The vascularized human placenta interfaces between the mother and fetus to transfer nutrients for sustaining pregnancy. Unlike that of adults, fetal high-density lipoprotein (HDL), which is in contact with placental vessels, is characterized by a high proportion of apolipoprotein E (apoE). We hypothesize this unique composition of fetal HDL affects key functions of ...[more]

PMID: 21954452

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