Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

32 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

An increase in the abundance of Tim23 in Arabidopsis thaliana results in the disruption of complex I and activation of mitochondrial biogenesis.

ABSTRACT: Analysis of changes in global transcript abundance profiles of 2 week old tim23 OE (overexpressor) and tim23 KO (knock-out) mutant Arabidopsis plants complared to wild-type (Col-0) using Affymetrix GeneChipﾙ Arabidopsis ATH1 Genome Arrays.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Estelle Giraud

PROVIDER: E-MEXP-3309 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Dual location of the mitochondrial preprotein transporters B14.7 and Tim23-2 in complex I and the TIM17:23 complex in Arabidopsis links mitochondrial activity and biogenesis.

Wang Yan Y Carrie Chris C Giraud Estelle E Elhafez Dina D Narsai Reena R Duncan Owen O Whelan James J Murcha Monika W MW

The Plant cell 20120622 6

Interactions between the respiratory chain and protein import complexes have been previously reported in Saccharomyces cerevisiae, but the biological significance of such interactions remains unknown. Characterization of two mitochondrial preprotein and amino acid transport proteins from Arabidopsis thaliana, NADH dehydrogenase B14.7 like (B14.7 [encoded by At2g42210]) and Translocase of the inner membrane subunit 23-2 (Tim23-2 [encoded by At1g72750]), revealed both proteins are present in respi ...[more]

PMID: 22730406

Similar Datasets

LETM2 is required for translation of mitochondrial encoded transcripts and is controlled by maternal epigenetic pathways in Arabidopsis thaliana

Project description:Arabidopsis ATH1 Genome Arrays were used to analyse changes in transcript abundance between Col-0 (wild-type) Arabidopsis seedlings and either single T-DNA insertional KO mutants of LETM1 (At3g59820)(T-DNA lines; SALK_067558C (letm1-1) and SALK_058471 (letm1-2)) or LETM2 (At1g65540) (T-DNA line; SALK_068877 (letm2-1)). Additionally, letm1 and letm2 knock out Arabidopsis lines were crossed to generate double mutants, however a double knock-out of these two genes results in an embryo lethal phenotype. Hemizygous plants were generated that were homozygous knock out for LETM1 and heterozygous knock out for LETM2, and visa versa, termed (letm1(-/-)LETM2(+/-) and (LETM1(+/-)letm2(-/-) respectively. Note that (letm1(-/-)LETM2(+/-) displays a mild developmental defective phenotype in the first 10-14 days of growth, while (LETM1(+/-)letm2(-/-) shows no phenotype. Microarray analysis was carried out on all three single homozygous knock out lines, and also on both combinations of the hemizygous mutation between the two genes, and compared with a wild-type Col-0 control to gain insight into global transcript abundance changes in these mutant lines. Arrays were performed in triplicate for each genotype, from RNA isolated from 3 independent pools of 5-10 Arabidopsis seedlings at 10 days old.

2012-06-01 | E-MEXP-3328 | biostudies-arrayexpress

Transcription profiling of SALK_084897 or SAIL_303_D08 Arabidopsis plants grown under normal conditions or with moderate light and drough treatment applied

Project description:4 week old Arabidopsis plants, of ecotype Columbia, SALK_084897 or SAIL_303_D08 were either grown under normal conditions or grown under normal conditions for before having a moderate light and drought treatment applied. Light and drought treatment was applied by withholding water for 5 days prior to transfer to 300 uE m-2 s-1 light conditions. Samples were collected after 3 days of treatment or for the same age plants grown under normal conditions.

2008-04-30 | E-ATMX-32 | biostudies-arrayexpress

ccr1-1

Project description:Regulation of carotenoid composition and shoot branching in Arabidopsis by a chromatin modifying histone methyltransferase, SDG8<br>Comparison of transcript profiles between wild type Columbia and ccr1 (carotenoid and chloroplast regulatory) mutant, which contains a mutation in At1g77300 (SDG8)

2009-10-01 | E-MEXP-1787 | biostudies-arrayexpress

nrb4 alleles

Project description:Plants of Arabidopsis thaliana (ecotype Col-0, nrb4-2 and nrb4-4) were grown in phytochambers in short day conditions during three weeks. Then, samples from different pots were mixed, and the RNA extracted.

2012-12-31 | E-MEXP-3602 | biostudies-arrayexpress

Transcription profiling of Arabidopsis dehydration on soil

Project description:Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07-1.08) under a 16 h light/8 h dark regimen (40 ± 10 ?mol photons/m2/s) at 22 C.Dehydration treatment: The 3-week-old plants were grown for 2 or 3 days without watering. To obtain accurate results, we carefully raised single plants in Petri dishes, each containing an equal amount of soil. Soil moisture contents were calculated from soil dry weight. Untreated; the soil moisture content was 84.3%. Under dehydration, on the second day, the soil moisture content was 51.1%. Under dehydration, on the third day, the moisture content was 11.6%.

2009-08-07 | E-MEXP-2174 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type Col-0 and hsf407 double knock out plants after heat stress

Project description:Effect of heat stress on wildtype COL-0 Arabidopsis and hsf4-7 double knock out plants.

2008-12-31 | E-MEXP-1725 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis plants after innoculation with isogenic Pseudomonas syringaie strains expressing one of four different avr genes

Project description:Gene expression profiles of a single Arabidopsis genotype (Col-0) in response to isogenic Pseudomonas syringae strains expressing one of four different cloned avr genes was studied (avrRpt2, avrRpm1, avrPphB, avrRps4; responses mediated by the R genes RPS2, RPM1, RPS5 and RPS4 ).

2008-02-29 | E-MEXP-1480 | biostudies-arrayexpress

Transcription profiling of AtHsp70-14 and AtHsp70-15 deficient Arabidopsis thaliana plants

Project description:AtHsp70-14 and -15 (At1g79930 and At1g79920) are two closely related isoforms of the Hsp110/SSE subfamily of the HSP70 chaperones in Arabidopsis thaliana. By analysing T-DNA insertion and amiRNA-AtHsp70-14/15 lines, we could demonstrate an essential function of AtHsp70-15 for plant growth. AtHsp70-15 deficient plants are reduced in size and stomata opening is impaired which results in accelerated wilting of detached leaves. Hsp70-15 deficient plants are also more tolerant to TuMV infection but more sensitive to heat treatment. Therefore comparative transcriptome analysis of amiRNA-AtHsp70-14/15, hsp70-14 knockout, hsp70-15 knockout and wild type plants was performed with leaf samples of 6 week old plants grown under ambient conditions. The analysis of differentially regulated genes compared to the wild type Col-0 plants exhibited a constitutive heat stress response / cytosolic protein response of Hsp70-15 deficient plants.

2011-03-28 | E-MEXP-3082 | biostudies-arrayexpress

Transcription profiling of Arabidopsis response to cold on soil

Project description:Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07-1.08) under a 16 h light/8 h dark regimen (40 ± 10 ?mol photons/m2/s) at 22 C. The light intensity was the same at 22 C and at 4 C. Plants were harvested 2 h later after lights were turned on. Cold treatment: The 3-week-old plants were transferred from 22 C to 4 C and were grown for 1 or 4 days.

2009-08-07 | E-MEXP-2173 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis plants treated either with mock or menadione sodium bisulphite and sampled after 3, 6 and 24 hours

Project description:Arabidopsis plants were treated either with mock or MSB (0.2 mM of Menadione sodium bisulphite). <br>Tissue was sampled after 3, 6 and 24 hours.

2009-09-11 | E-MEXP-1934 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data