Affymetrix expression arrays from heart extracted RNA in Ts65Dn and Ms5Yah and Ts65DnMs5Yah mouse models
Ontology highlight
ABSTRACT:
N = 5 mices per group of genotype.
Goal : assess the gene dosage effect on partial trisomic/monosomic mice modelling human Down syndrome.
ORGANISM(S): Mus musculus
SUBMITTER: HERAULT Yann
PROVIDER: E-MEXP-3355 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
The App-Runx1 region is critical for birth defects and electrocardiographic dysfunctions observed in a Down syndrome mouse model.
Raveau Matthieu M Lignon Jacques M JM Nalesso Valérie V Duchon Arnaud A Groner Yoram Y Sharp Andrew J AJ Dembele Doulaye D Brault Véronique V Hérault Yann Y
PLoS genetics 20120531 5
Down syndrome (DS) leads to complex phenotypes and is the main genetic cause of birth defects and heart diseases. The Ts65Dn DS mouse model is trisomic for the distal part of mouse chromosome 16 and displays similar features with post-natal lethality and cardiovascular defects. In order to better understand these defects, we defined electrocardiogram (ECG) with a precordial set-up, and we found conduction defects and modifications in wave shape, amplitudes, and durations in Ts65Dn mice. By using ...[more]
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Project description:Affymetrix expression arrays from hippocampus of Ms2Yah mouse model of Down syndrome
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Project description:To understand the molecular basis of Down syndrome pathogenesis, we performed a transcriptome analysis of nine different tissues in Ts65Dn, an established mouse model of human trisomy 21. Ts65Dn mice have segmental trisomy of mouse chromosome 16 with ca. 128 genes at dosage imbalance (Reeves et al. 1995). The Ts65Dn mouse is widely used as a model for studies of DS because it is at dosage imbalance for the orthologs of about half the 284 Chr21 genes. Ts65Dn mice have several features that directly parallel developmental anomalies of DS. We compare here the expression of 136 mouse orthologs of Chr21 genes, 77 of which are triplicated in Ts65Dn, in trisomic and euploid mice. <br> <br> We designed a mouse cDNA expression array interrogating 136 mmu21 genes. RNA pools from four adult male Ts65Dn mice and four male euploid littermates were prepared from cortex and dissected from three to four month-old mice. Directly labeled first strand cDNA probes from nine different tissues were hybridized to the arrays in replicated hybridizations. A total of 446 genes that are not triplicated in Ts65Dn mice served as controls. These included 62 mmu21 genes from MMU10, MMU17, and non-triplicated portions of MMU16, plus 384 randomly distributed mouse cDNAs from the Unigene collection.
2004-04-23 | E-MEXP-102 | biostudies-arrayexpress
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2009-12-30 | E-TABM-609 | biostudies-arrayexpress
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