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Microarray gene expression profiling in E2f7 and E2f8 deleted liver compared to control in mice

ABSTRACT: Two condition experiment were compared: Wild type vs. E2f7/8 dko mouse liver. Biological replicates: two wild type replicates and two E2f7/8 dko replicates for each of 3-wk, 4-wk and 16-wk age groups.

ORGANISM(S): Mus musculus

SUBMITTER: Shusil Pandit

PROVIDER: E-MEXP-3384 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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E2F8 is essential for polyploidization in mammalian cells.

Pandit Shusil K SK Westendorp Bart B Nantasanti Sathidpak S van Liere Elsbeth E Tooten Peter C J PC Cornelissen Peter W A PW Toussaint Mathilda J M MJ Lamers Wouter H WH de Bruin Alain A

Nature cell biology 20121014 11

Polyploidization is observed in all mammalian species and is a characteristic feature of hepatocytes, but its molecular mechanism and biological significance are unknown. Hepatocyte polyploidization in rodents occurs through incomplete cytokinesis, starts after weaning and increases with age. Here, we show in mice that atypical E2F8 is induced after weaning and required for hepatocyte binucleation and polyploidization. A deficiency in E2f8 led to an increase in the expression level of E2F target ...[more]

PMID: 23064264

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E2f7/E2f8 and E2f1/E2f2/E2f3 null and wild type liver along with E2f7/E2f8 null and wild type trophoblast giant cells

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Expression data from E2f7/E2f8/E2f3a null placentas and embryos

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Project description:To understand the underlying cause and mechanisms of embryonic lethality observed in combined loss of E2f7 and E2f8, we compared global gene expression profiles of wild type, germline deleted and sox2-Cre/Cyp19-Cre deleted embryos and placentas. RNA was extracted from E10.5 embryos and placentas. Gene deletion in samples confirmed by PCR genotyping of DNA isolated from each sample.

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Expression data from E2f7/E2f8 and E2f1/E2f2/E2f3 null liver (Affymetrix)

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Expression data from E2f7/E2f8 and E2f1/E2f2/E2f3 null liver (Affymetrix)

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2012-08-31 | E-GEOD-32354 | biostudies-arrayexpress

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| S-EPMC2267382 | biostudies-literature

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