Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

47 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Myc cell types

ABSTRACT: cortical cell of non-mycorrhizal roots (cor), arbuscule-containing cells (arb) and non-arbuscule-containing cells (nac) of M. truncatula roots colonized with Glomus intraradices were collected by laser capture microdissection (LCM) and used for RNA extraction and Medicago microarray hybridisation

ORGANISM(S): Medicago truncatula

SUBMITTER: Franziska Krajinski

PROVIDER: E-MEXP-3385 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of arbuscular mycorrhiza established at 4 weeks post inoculation between Medicago truncatula and Glomus mosseae

Project description:The transcriptome profile of arbuscular mycorrhiza established at 4 weeks post inoculation between Medicago truncatula and Glomus mosseae as well as between Medicago truncatula and Glomus intraradices is compared

2005-06-30 | E-MEXP-218 | biostudies-arrayexpress

Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

Project description:Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel gene expression in Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on whole mycorrhizal roots. We used GeneChips to detail the global programme of gene expression in response to colonization by arbuscular mycorrhizal fungi and in response to a treatment with phosphate and identified genes differentially expressed during this process. Medicago truncatula roots were harvested at 28 days post inoculation with the two different arbuscular mycorrhizal fungi Glomus intraradices (Gi-Myc) and Glomus mosseae (Gm-Myc) under low phosphate conditions (20 µM phosphate) or after a 28 days treatment with 2 mM phosphate in the absence of arbuscular mycorrhizal fungi (2mM-P). As a control, uninfected roots grown under low phosphate conditions (20 µM phosphate) were used (20miM-P). Three biological replicates consisting of pools of five roots were used for RNA extraction and hybridization on Affymetrix GeneChips.

2011-10-28 | E-GEOD-32208 | biostudies-arrayexpress

Small RNA sequencing in Medicago truncatula roots (Glomus intraradices colonized and non-colonized)

Project description:Identification of microRNA expressed in M. truncatula roots Small RNA sequencing of mycorrhizal and non-mycorrhiza M. truncatula roots

2011-06-16 | E-GEOD-26216 | biostudies-arrayexpress

Degradome sequencing in Medicago truncatula roots (Glomus intraradices colonized and non-colonized)

Project description:Identification of microRNA targets in M. truncatula roots Degradome sequencing of mycorrhizal and non-mycorrhiza M. truncatula roots

2011-06-16 | E-GEOD-26217 | biostudies-arrayexpress

A roadmap of cell-type specific gene expression during late stages of the arbuscular mycorrhiza symbiosis

Project description:Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel cell-type specific gene expression during late stages of Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on laser-microdissected cells. We used Medicago GeneChips to detail the cell-type specific programme of gene expression in late stages of colonization by arbuscular mycorrhizal fungi and identified genes differentially expressed during these stages. Medicago truncatula Gaertn M-bM-^@M-^XJemalongM-bM-^@M-^Y genotype A17 plantlets were grown in the climate chamber. Plants grown for the collection of root cortical cells containing arbuscules (ARB), root cortical cells from mycorrhizal roots (CMR), and root epidermal cells from mycorrhizal roots (EPI) were mycorrhized after 2 weeks with Glomus intraradices and mycorrhizal roots were harvested at around 21 days post inoculation (dpi).

2013-04-28 | E-GEOD-42747 | biostudies-arrayexpress

Substantial equivalence in transgenic barley

Project description:In this study, possible adverse effects of transgene expression in field-grown barley were assessed in relation to the influence of genetic background and to the impact of interaction with arbuscular mycorrhiza fungi. The deposited microarray data originate from a parallel transcript profiling, metabolome profiling and metabolic fingerprinting approach with the wild type cultivars Golden Promise (GP) and Baronesse (B) as well as barley transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the Golden Promise (GP) into the cultivar Baronesse (B) and ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). Our conclusion from the results is that cultivar-specific differences and even the presence of few introgressed alleles exceed the effects on transcriptome and metabolome caused by transgene expression. Mycorrhization was shown to have more prominent effects on the metabolome than on the transcriptome. Hordeum vulgare plants were cultivated in the field in 2007 at Giessen Experimental station (165m elevation over NN, 1540h sunshine and 650mm precipitation per year in average) in replicated blocks, each consisted of eight 0.8 m × 0.5 m plots in which genotypes were randomly distributed and half of the plots were pretreated with Amykor® (containing the arbuscular fungi Glomus intraradices and Glomus mosseae). Ten pools of leaf material of at least 10 leaves per pool were sampled in the middle of the light period four months after planting. Two replicates of each genotype were subjected to microarray analysis.

2010-03-02 | E-GEOD-19296 | biostudies-arrayexpress

Gene profiling in arbuscular mycorrhizal symbiosis between the actinorhizal tree Casuarina glauca and the fungus Glomus intraradices.

Project description:Casuarina glauca belongs to a family of angiosperms called actinorhizal plants because they can develop nitrogen-fixing nodules in association with the soil bacteria Frankia. They can also develop arbuscular mycorrhizae (AM) while associated with Glomeromycota fungi. The aim of this transcriptomic study was to get a global view of the plant symbiotic genetic program in AM and to identify new key plant genes involved in endosymbioses. C. glauca plants were grown in hydroponics then transferred to pots with or without spores of Glomus intraradices and watered with low phosphate solution to enhance mycorrhization. For this study we considered two stages: - a stage where plants were inoculated with G. intraradices, roots were harvested 8 weeks after inoculation with the G. intraradices. AM structures were present. - a stage where plants were not inoculated, roots were harvested at the same time as the inoculated roots , this is our control condition. AM structures were absent. Three biological replicates were used for each condition. Microarrays were designed by Imaxio (Clermont Ferrand, France ; http://www.imaxio.com/index.php) which has been accredited by Agilent Technologies (Palo Alto, CA, USA; http://www.home.agilent.com/agilent/home.jspx) as a certified service provider for microarray technologies. Based on 14327 annotated unigenes for C. glauca, 60mers probes were designed using eArray software (1 probe per unigene) and custom 8 x 15K Oligo Microarrays were manufactured by Agilent.

2012-10-22 | E-GEOD-36676 | biostudies-arrayexpress

Transcription profiling of Lycopersicon esculentum roots in wild-type and ABA-deficient, control and Glomus intraradices inoculated plants to characterise variations in mycorrhization.

Project description:Abscisic acid (ABA) determines mycorrhiza functionality and arbuscule development. Transcriptome analysis in response to different mycorrhization status according to the ABA concentration in the root was performed to identify genes that may play a role in arbuscule functionality. Tomato Affymetrix GeneChip (around 10,000 probes) allowed us to detect and compare the transcriptional root profiling of tomato (Solanum lycopersicum) wild-type and ABA-deficient sitiens plants colonized by the arbuscular mycorrhizal fungus Glomus intraradices. <br><br>

2010-07-01 | E-MEXP-2356 | biostudies-arrayexpress

Arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long-term virus accumulation

Project description:Transcriptional changes triggered by the systemic infection of the tospovirus Tomato Spotted Wilt Virus (TSWV) in roots and shoots of tomato plants (Solanum lycopersicum) mycorrhized by Glomus mosseae

2011-10-31 | E-MTAB-620 | biostudies-arrayexpress

Transcription profiling of Physcomitrella patens response to fungal exudates

Project description:Physcomitrella patens gametophores were treated with exudates from the arbuscular mycorrhiza fungi (AMF) Rhizophagus irregularis (formerly known as Glomus intraradices) and Gigaspora margerita for one hour and 24 hours.

2014-11-10 | E-MTAB-3081 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data