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Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol-acetone ratios

ABSTRACT: Artificial electron carriers have been widely used to shift the solvent ratio towards butanol in acetone-butanol-ethanol (ABE) fermentation of solventogenic clostridia according to decreased hydrogen production. In this study, first insights on the molecular level were gained to explore the effect of methyl viologen addition to cultures of Clostridium acetobutylicum. Employing batch fermentation in mineral salts medium, the butanol:acetone ratio was successively increased from 2.3 to 12.4 on a 100 ml scale in serum bottles and from 1.4 to 16.5 on a 1,300 ml scale in bioreactors, respectively. The latter cultures were used for DNA microarray analyses to provide new information on the transcriptional changes referring to methyl viologen exposure and thus, exhibing gene expression patterns according to the manipulation of the cellular redox balance.

ORGANISM(S): Clostridium acetobutylicum

SUBMITTER: Holger Janssen

PROVIDER: E-MEXP-3417 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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