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Kdm2b facilitates iPS (induced pluripotent stem cells) cell generation

ABSTRACT: RNA samples were extracted from mouse embryonic fibroblast transduced with Oct4, Sox2 and Klf4 (OSK) or OSK plus Kdm2b at post-transduction day 4, 8 and 12.

ORGANISM(S): Mus musculus

SUBMITTER: Gaoyang Liang

PROVIDER: E-MEXP-3433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Kdm2b promotes induced pluripotent stem cell generation by facilitating gene activation early in reprogramming.

Liang Gaoyang G He Jin J Zhang Yi Y

Nature cell biology 20120422 5

Transcription-factor-directed reprogramming from somatic cells to induced pluripotent stem cells (iPSCs) is by nature an epigenetic process of cell fate change. Previous studies have demonstrated that this inefficient process can be facilitated by the inclusion of additional factors. To gain insight into the reprogramming mechanism, we aimed to identify epigenetic enzymes capable of promoting iPSC generation. Here we show that Kdm2b, a histone H3 Lys 36 dimethyl (H3K36me2)-specific demethylase, ...[more]

PMID: 22522173

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2013-02-20 | E-GEOD-41298 | biostudies-arrayexpress

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2016-03-01 | E-GEOD-66798 | biostudies-arrayexpress

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Global Transcriptional Analysis of Nuclear Reprogramming in the Transition from MEFs to iPSCs

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2012-10-01 | E-GEOD-37397 | biostudies-arrayexpress

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2012-12-26 | GSE40701 | GEO

Gene expression changes following knockdown of Kdm2b on mESCs

Project description:In order to study the effects of Kdm2b binding at CpG islands, Kdm2b was knocked down in mouse embryonic stem cells using shRNA and gene expression profiled using Affymetrix arrays Depletion of KDM2B affects the gene expression of a subset of polycomb targets RNA from 4 samples stably expressing shKdm2b and 4 samples stably expressing a control shRNA was hybridized to Affymetrix Mouse Gene 1.0 ST Arrays

2012-12-26 | E-GEOD-40701 | biostudies-arrayexpress

The histone demethylase KDM2B regulates human primordial germ cell-like cells specification

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2021-01-10 | GSE160287 | GEO

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