Transcription profiling of C57/BL6J mice at various ages post partum vs a common control adult (8 week) testis RNA from the same strain

ABSTRACT: Testis RNA from C57/BL6J mice at various ages post partum was compared to a common control consisting of adult (8 week) testis RNA from the same strain. This generates a time course showing the activation of genes on the array across the first wave of spermatogenesis, which can then be correlated with the appearance of specific germ cell types, allowing assignation of unknown genes to differing cell types and charting the evolution of the developing testis transcriptome. Adult testis RNA from six different genetic models of infertility (XXSxrb, mshi, Bax -/-, bs, Bcl-w -/- and azh) were compared to age- and strain-matched normal control testis RNA. These models possess different cellular complements within the testis, which can be interpreted within the framework established by the first wave analysis and used to refine the assignment of genes to cell type.

ORGANISM(S): Mus musculus

DISEASE(S): These mice have a relatively normal first wave of spermatogenesis but never become fertile. Older animals show increased apoptosis of germ cells, increasing in severity as germ cell stage advances.

SUBMITTER: Peter Ellis 

PROVIDER: E-MEXP-35 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress