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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription Profiling of the Persister Phenotype in Escherichia Coli

ABSTRACT: When bacteria are challenged with antimicrobials, small numbers of cells may survive treatment. These so-called persister cells are not mutants, but are a set of phenotypic variants which, after re-growth exhibit the same susceptibility properties as their progenitor populations. The presence of persisters may determine the effective therapeutic dose of antibiotics and account for the intractability of biofilm-related infections. In the current investigation, Escherichia coli persister cells were isolated without prior exposure to antimicrobials, using fluorescence-assisted cell sorting of a strain, labelled with a chromosomal green fluorescent protein marker. Levels of persistence were inversely proportional to fluorescence intensity in the gfp-tagged E. coli population, and separated persister cells showed enhanced expression of two groups of genes located in the e14 and CP4-6 cryptic prophage regions when analysed by DNA microarray.

ORGANISM(S): Escherichia coli

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-3511 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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