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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Cellular responses to the oxidizing effects of bleach

ABSTRACT: E. coli MG1655 was grown in MOPS minimal glucose medium at 37 degrees C with aeration to an OD600 of 0.4 - 0.5, and HOCl was added to a final concentration of 400 ?M. 0.5 ml samples were collected in liquid nitrogen immediately before, 5 min after, and 10 min after HOCl addition, and total RNA was prepared using the RNeasy? Midi kit (Qiagen). cDNA synthesis, array hybridization to Affymetrix GeneChip E. coli genome 2.0 Arrays, and imaging were performed according to Affymetrix guidelines at the Affymetrix and Microarray Core facility at the University of Michigan, Ann Arbor.

ORGANISM(S): Escherichia coli

SUBMITTER: Wei-Yun Wholey

PROVIDER: E-MEXP-3639 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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NemR is a bleach-sensing transcription factor.

Gray Michael J MJ Wholey Wei-Yun WY Parker Benjamin W BW Kim Minwook M Jakob Ursula U

The Journal of biological chemistry 20130327 19

<h4>Background</h4>Little is known about how bacteria sense or respond to reactive chlorine species, such as bleach.<h4>Results</h4>NemR is a redox-regulated transcription factor which senses bleach.<h4>Conclusion</h4>NemR controls expression of genes encoding electrophile detoxification enzymes, which increase bleach resistance.<h4>Significance</h4>We demonstrate a bleach-sensing bacterial response system and a new mechanism contributing to bacterial bleach survival. Hypochlorous acid (HOCl), t ...[more]

PMID: 23536188

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