Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of genetically cerebral malaria-resistant (CM-R) and cerebral malaria-susceptible (CM-S) mouse strains

ABSTRACT: Gene expression patterns were investigated in well-defined genetically cerebral malaria-resistant (CM-R) and cerebral malaria-susceptible (CM-S) mouse strains. cDNA microarrays were used to search for differentially expressed genes in mouse brain. Four mouse strains, known to differ in susceptibility to cerebral malaria upon Plasmodium berghei ANKA infection, were compared: BALB/c and DBA/2 mice are CM-R, while C57BL/6 and CBA/J mice are CM-S.

INSTRUMENT(S): FUJIFILM BAS-5000 scanning hardware

ORGANISM(S): Mus musculus

SUBMITTER: Nicolas Coltel

PROVIDER: E-MEXP-370 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene-expression profiling discriminates between cerebral malaria (CM)-susceptible mice and CM-resistant mice.

Delahaye Nicolas F NF Coltel Nicolas N Puthier Denis D Flori Laurence L Houlgatte Remi R Iraqi Fuad A FA Nguyen Catherine C Grau Georges E GE Rihet Pascal P

The Journal of infectious diseases 20051205 2

The development of cerebral malaria (CM) in mice with Plasmodium berghei ANKA infection is under genetic control. Brain gene-expression patterns were investigated in well-defined genetically CM-resistant (CM-R; BALB/c and DBA/2) and CM-susceptible (CM-S; C57BL/6 and CBA/J) mice by use of cDNA microarrays. By combining transcriptional profiling with rigorous statistical methods and cluster analysis, we identified a set of 69 genes that perfectly discriminated between mouse strains and between CM- ...[more]

PMID: 16362897

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Project description:The antenno-maxilary complex (AMC) forms the chemosensory system of the Drosophila larva and is involved in gustatory and olfactory perception. We have previously shown that a mutant allele of the homeodomain transcription factor Prospero (prosVoila1, V1), presents several developmental defects including abnormal growth and altered taste responses. In addition, many neural tracts connecting the AMC to the central nervous system (CNS) were affected. Our earlier reports on larval AMC did not argue in favour of a role of pros in cell fate decision, but strongly suggested that pros could be involved in the control of other aspect of neuronal development. In order to identify these functions, we used microarray analysis of larval AMC and CNS tissue isolated from the wild type, and three other previously characterised prospero alleles, including the V1 mutant, considered as a null allele for the AMC. A total of 17 samples were first analysed with hierarchical clustering. To determine those genes affected by loss of pros function, we calculated a discriminating score reflecting the differential expression between V1 mutant and other pros alleles. We identified a total of 64 genes in the AMC. Additional manual annotation using all the computed information on the attributed role of these genes in the Drosophila larvae nervous system, enabled us to identify a first functional category of potential Prospero target genes known to be involved in neurite outgrowth, synaptic transmission and more specifically in neuronal connectivity remodelling. The second category of genes found to be differentially expressed between the null mutant AMC and the other alleles concerned the development of the sensory organs and more particularly the larval olfactory system. Surprisingly, a third category emerged from our analyses and suggests an association of pros with the genes that regulate autophagy, growth and insulin pathways. Interestingly, EGFR and Notch pathways were represented in all of these three functional categories. We now propose that Pros could perform all of these different functions through the modulation of these two antagonistic and synergic pathways. The current data contribute to the clarification of the Prospero function in the larval AMC and show that pros regulates different function in larvae as compared to those controlled by this gene in embryos. In the future, the possible mechanism by which Pros could achieve its function in the AMC and the possible involvement of EGFR and Notch pathway will be explored in detail Gene expression was measured in two different tissues (Brain and AMC) of prospero mutants flies (V1, V13, V14, V24). 3 biological replicates were performed for V1, V13 and V24 AMC and V14 Brain; 2 biological replicates for V14 AMC and V13 Brain.

Dataset Information

Transcription profiling of genetically cerebral malaria-resistant (CM-R) and cerebral malaria-susceptible (CM-S) mouse strains

Publications

Gene-expression profiling discriminates between cerebral malaria (CM)-susceptible mice and CM-resistant mice.

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