Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Hepatic transcription profiling of mice exposed to As and Fe by drinking water

ABSTRACT: Five-week-old male mice (Mus musculus, ICR) were exposed to single As, single Fe and combined As and Fe for 90 days. A total of 40 mice were randomly assigned to control and three metal-treated groups. Nine mice were applied in every group. For control group, mice were fed with pure water. For three metal-treated groups, mice were feed with 3mg/L As, 5mg/L Fe and 3mg/L As + 5mg/L Fe, respectively. After exposure, mice were anaesthetized under isoflurane followed by exsanguination. Livers were removed, and hepatic RNA for each mouse was immediately extract. The gene expression profiles for control and three metal-treated groups were determined by the GeneChip Mouse Gene 1.0 ST arrays. Biological meanings of DEGs were analyzed by pathway analysis. DEGs were mapped to different biological pathways according to Kyoto encyclopedia of genes and genomes (KEGG) pathway database (http://www.genome.ad.jp/kegg/ pathway.html) using SBC Analysis System of Shanghai Biotechnology Corporation (http://sas.ebioservice.com/).

ORGANISM(S): Mus musculus

SUBMITTER: Bing Wu

PROVIDER: E-MEXP-3763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Impact of iron precipitant on toxicity of arsenic in water: a combined in vivo and in vitro study.

Liu Su S Guo Xuechao X Zhang Xuxiang X Cui Yibin Y Zhang Yan Y Wu Bing B

Environmental science & technology 20130320 7

Removing arsenic (As) from drinking water is widely dependent on iron (Fe)-based coagulation/flocculation techniques. However, little is known about the influence of Fe precipitant on As toxicity. In this present study, the influence of Fe on As toxicity was determined at systems biology level by in vitro and in vivo experiments. In vitro study based on HepG2 cell line found that Fe increased the As toxicity on cell viability and DNA damage, indicating the synergetic toxic effects. However, when ...[more]

PMID: 23473362

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Project description:Essential metals such as iron are required for healthy plant growth. Fe is an important cofactor and catalytic element in many biological processes. Fe and other metals can also be toxic when present in excess. Therefore plants have mechanisms of metal homeostasis which involve coordination of metal ion transporters for uptake, translocation and compartmentalisation. The NAS genes are supposed to play an important role in Fe homeostasis. They are coding for enzymes called nicotianaminesynthase (NAS), which synthesize nicotianamine (NA) by a one-step condensation reaction of three molecules S-adenosyl-methionine. NA acts as a chelator for Fe, Cu, Ni and Zn and might be involved in the transport and allocation of Fe throughout the plant. We generated quadruple T-DNA insertion mutant nas plants to investigate NA function as described in Klatte et al., 2009, Plant Physiol. The nas4x-1 plants show an interveinal leaf chlorosis when turning from vegetative to reproductive stage, which intensifies when growing under Fe deficiency conditions. nas4x-1 plants have strongly reduced NA contents and show an elevated Fe deficiency response in roots. By performing microarray experiments we want to reveal global changes on transcriptional level in roots and leaves of nas4x-1 mutant compared to wild type plants grown under Fe supply and Fe deficiency conditions, respectively. The loss of NAS genes has a strong impact on the regulation of other metal homeostasis genes and allows to draw conclusions about nicotianamine function in metal homeostasis of A.thaliana. For this study, four-week old nas4x-1 mutant and wild type plants were exposed for 7 days to plant medium with and without Fe supply. These conditions have been established previously and have resulted in a reproducibly strong interveinal leaf chlorosis of nas4x-1 plants compared to wild type, especially upon Fe deficiency conditions. The experiment was repeated three times in consecutive weeks to obtain three independent biological repetitions. Rosette leaves and roots of five week-old plants were harvested, RNA was isolated and microarray hybridization was performed. 24 Total samples were analyzed. We generated the following pairwise comparisons: WT + Fe vs. – Fe, nas4x-1 + Fe vs. – Fe, + Fe WT vs. + Fe nas4x-1, - Fe WT vs. - Fe nas4x-1, roots and leaves

Dataset Information

Hepatic transcription profiling of mice exposed to As and Fe by drinking water

Publications

Impact of iron precipitant on toxicity of arsenic in water: a combined in vivo and in vitro study.

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