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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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S. pneumoniae R6 LVX

ABSTRACT: An exponentially growing culture of strain R6 in AGCH at OD620nm=0.4 was either non-treated or treated with two LVX concentrations: 0.125 ug/ml LVX (0.5x MIC of R6) and 2.5 ug/ml LVX (10x MIC of R6). Samples were taken before treatment (0 min), at 15, 30 and 60 min in the non-treated culture, and at 5, 15 ,30 and 60 min in the LVX treated cultures

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Adela de la Campa

PROVIDER: E-MEXP-3809 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The fluoroquinolone levofloxacin triggers the transcriptional activation of iron transport genes that contribute to cell death in Streptococcus pneumoniae.

Ferrándiz María-José MJ de la Campa Adela G AG

Antimicrobial agents and chemotherapy 20131021 1

We studied the transcriptomic response of Streptococcus pneumoniae to levofloxacin (LVX) under conditions inhibiting topoisomerase IV but not gyrase. Although a complex transcriptomic response was observed, the most outstanding result was the upregulation of the genes of the fatDCEB operon, involved in iron (Fe(2+) and Fe(3+)) uptake, which were the only genes varying under every condition tested. Although the inhibition of topoisomerase IV by levofloxacin did not have a detectable effect in the ...[more]

PMID: 24145547

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