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Transcription profiling of human embryonic kidney cells expressing apoptosis inducing proteins, time series

ABSTRACT: Three apoptosis inducing proteins, ACO1, STK3 and XBP1 were discovered in a previous reverse transfection screen. HEK293T cells were harvested and RNA extracted from the cells after over-expression of these 3 proteins at 12, 24 and 48 hours. A positive apoptosis inducer staurosporine and a negative control transfection reagent only were also included at each time point. Each sample had an experimental replicate.

ORGANISM(S): Homo sapiens

SUBMITTER: Ella Palmer

PROVIDER: E-MEXP-421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays.

Yap Yee Leng YL Lam David C L DC Luc Girard G Zhang Xue Wu XW Hernandez David D Gras Robin R Wang Elaine E Chiu S W SW Chung Lap Ping LP Lam W K WK Smith David K DK Minna John D JD Danchin Antoine A Wong Maria P MP

Nucleic acids research 20050114 1

Gene transcription in a set of 49 human primary lung adenocarcinomas and 9 normal lung tissue samples was examined using Affymetrix GeneChip technology. A total of 3442 genes, called the set M AD, were found to be either up- or down-regulated by at least 2-fold between the two phenotypes. Genes assigned to a particular gene ontology term were found, in many cases, to be significantly unevenly distributed between the genes in and outside M AD. Terms that were overrepresented in M AD included func ...[more]

PMID: 15653641

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2010-07-01 | E-MTAB-162 | biostudies-arrayexpress

Transcription profiling by array of human serum-free fibrocytes, serum-containing fibrocytes, CD14++CD16- Monocytes, CD14++CD16+ Monocytes, CD14+CD16++ Monocytes and macrophages

Project description:Serum-free Fibrocytes, Serum-containing Fibrocytes, CD14++CD16- Monocytes, CD14++CD16+ Monocytes, CD14+CD16++ Monocytes, Macrophages were all generated from up to 3 biological replicates from each of 3 separate donors. RNA was extracted (Ambion RNAqueous), labelled with cy3, mixed with cy5 labelled human reference (Stratagene), and hybridised to slides printed with Human AROS v4.0 oligonucleotides (Operon). Slides were scanned using a Perkin Elmer GX plus, and the data then normalised with GEPAS v4.0 and collated. Final data analysis was carried out using TMEV 4.0. SAM was performed using a 0.1% FDR. PCA were plotted from this list, and interrogation carried out using DAVID to determine pathway enrichment.

2011-01-01 | E-MEXP-2811 | biostudies-arrayexpress

Transcription profiling of human CD4 regulatory T cells that express either CD45RA or CD45RO

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2009-10-05 | E-MEXP-2372 | biostudies-arrayexpress

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2010-04-29 | E-MEXP-2434 | biostudies-arrayexpress

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2007-06-19 | E-MEXP-795 | biostudies-arrayexpress

Transcription profiling of wild type and atNRT2.7 genotype Arabidopsis leaves and roots grown in high or low nitrate conditions

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2007-07-01 | E-MEXP-801 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thioredoxin reductase knock-out mutant

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2005-09-15 | E-MEXP-365 | biostudies-arrayexpress

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2009-06-03 | E-MEXP-1910 | biostudies-arrayexpress

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