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Transcription profiling of mouse cells expressing tet inducible LhX2, withdrawal of LHX2 over time was analysed

ABSTRACT: Expression of the lim-homeodomain transcription factor is required for sustained maintenance of heamatopoietic stem cell like cells in undifferentiated form durng in vitro culturing. Cell lines were created from the mouse embryonic stem (ES) cell line, Ainv15. The gene Lhx2 was introducted into these cells under the control of a tetracycline-responsive element. In the presence of tetracycline (or its analogue doxycycline), these cells express Lhx2. In DoxHPC7 GFP is co-expressed together with the Lhx2 gene. We used these cells to carry out a time-course study where the effects of Lhx2 withdrawal were studied.

INSTRUMENT(S): G2565BA Microarray Scanner

ORGANISM(S): Mus musculus

SUBMITTER: Joakim Lunderberg

PROVIDER: E-MEXP-431 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global gene expression analyses of hematopoietic stem cell-like cell lines with inducible Lhx2 expression.

Richter Karin K Wirta Valtteri V Dahl Lina L Bruce Sara S Lundeberg Joakim J Carlsson Leif L Williams Cecilia C

BMC genomics 20060406

<h4>Background</h4>Expression of the LIM-homeobox gene Lhx2 in murine hematopoietic cells allows for the generation of hematopoietic stem cell (HSC)-like cell lines. To address the molecular basis of Lhx2 function, we generated HSC-like cell lines where Lhx2 expression is regulated by a tet-on system and hence dependent on the presence of doxycyclin (dox). These cell lines efficiently down-regulate Lhx2 expression upon dox withdrawal leading to a rapid differentiation into various myeloid cell t ...[more]

PMID: 16600034

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