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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse control 25-dpc embryos from control vs. valproic acid (VPA) treated dams and control P19 mouse EC cells vs 1 mM sodium valproate for 24 h

ABSTRACT: Valproic acid (VPA) is a potent inducer of neural tube defects (NTDs), but its mechanism of teratogenicity is not known. To study the transcriptional response of VPA during the susceptible period, i.e. when VPA is likely to exert most of its teratogenic effect, RNA was extracted from 8.25-dpc embryos (6 h post treatment) from control and VPA-treated dams, and subjected to microarray analysis (four arrays). To be more useful for risk assessment, in vitro models, using cells, may bridge biomarkers discovered by gene expression profiling in an animal in vivo model. Since pluripotent embryocarcinoma (EC) cell lines may be relevant models for early embryonic cells, P19 mouse EC cells were treated with or without 1 mM sodium valproate for 24 h and alos subjected to microarray analysis (four arrays).

ORGANISM(S): Mus musculus

SUBMITTER: Kim Kultima

PROVIDER: E-MEXP-45 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Valproic acid teratogenicity: a toxicogenomics approach.

Kultima Kim K Nyström Anna-Maja AM Scholz Birger B Gustafson Anne-Lee AL Dencker Lennart L Stigson Michael M

Environmental health perspectives 20040801 12

Embryonic development is a highly coordinated set of processes that depend on hierarchies of signaling and gene regulatory networks, and the disruption of such networks may underlie many cases of chemically induced birth defects. The antiepileptic drug valproic acid (VPA) is a potent inducer of neural tube defects (NTDs) in human and mouse embryos. As with many other developmental toxicants however, the mechanism of VPA teratogenicity is unknown. Using microarray analysis, we compared the global ...[more]

PMID: 15345369

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Transcription profiling of mouse embryos 1.5, 3 and 6 hr after maternal treatment with the teratogen sodium valproate

Project description:The antiepileptic drug valproic acid (vpa) is known teratogen giving neural tube defects (ntd:s). Administration of vpa to female NMRI on gestation day 8 induces a high incidence of ntd. In this study we investigate the time dependent gene expression changes induced in the embryo 1.5, 3 respectively 6 hr after maternal sodium valproate treatment.

2008-03-28 | E-MEXP-1062 | biostudies-arrayexpress

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Analysis of total proteome changes upon long-term treatment with Rho kinase inhibitor H1152.

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Israeli acute paralysis virus – what we have learned about the virus since mysterious disappearance of the US honey bees known as Colony Collapse Disorder (CCD) in 2006

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Broad epigenetic signature of maternal care in the brain of adult rats (expression)

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